Cargando…
Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698364/ https://www.ncbi.nlm.nih.gov/pubmed/36475567 http://dx.doi.org/10.1016/j.xpro.2021.101041 |
_version_ | 1784838797572177920 |
---|---|
author | Pankaew, Saran Grosjean, Clémence Quessada, Julie Loosveld, Marie Potier, Delphine Payet-Bornet, Dominique Nozais, Mathis |
author_facet | Pankaew, Saran Grosjean, Clémence Quessada, Julie Loosveld, Marie Potier, Delphine Payet-Bornet, Dominique Nozais, Mathis |
author_sort | Pankaew, Saran |
collection | PubMed |
description | Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). |
format | Online Article Text |
id | pubmed-9698364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96983642022-11-26 Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples Pankaew, Saran Grosjean, Clémence Quessada, Julie Loosveld, Marie Potier, Delphine Payet-Bornet, Dominique Nozais, Mathis STAR Protoc Protocol Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). Elsevier 2021-12-20 /pmc/articles/PMC9698364/ /pubmed/36475567 http://dx.doi.org/10.1016/j.xpro.2021.101041 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Pankaew, Saran Grosjean, Clémence Quessada, Julie Loosveld, Marie Potier, Delphine Payet-Bornet, Dominique Nozais, Mathis Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples |
title | Multiplexed single-cell RNA-sequencing of mouse thymic and splenic
samples |
title_full | Multiplexed single-cell RNA-sequencing of mouse thymic and splenic
samples |
title_fullStr | Multiplexed single-cell RNA-sequencing of mouse thymic and splenic
samples |
title_full_unstemmed | Multiplexed single-cell RNA-sequencing of mouse thymic and splenic
samples |
title_short | Multiplexed single-cell RNA-sequencing of mouse thymic and splenic
samples |
title_sort | multiplexed single-cell rna-sequencing of mouse thymic and splenic
samples |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698364/ https://www.ncbi.nlm.nih.gov/pubmed/36475567 http://dx.doi.org/10.1016/j.xpro.2021.101041 |
work_keys_str_mv | AT pankaewsaran multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT grosjeanclemence multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT quessadajulie multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT loosveldmarie multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT potierdelphine multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT payetbornetdominique multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples AT nozaismathis multiplexedsinglecellrnasequencingofmousethymicandsplenicsamples |