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Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples

Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages...

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Autores principales: Pankaew, Saran, Grosjean, Clémence, Quessada, Julie, Loosveld, Marie, Potier, Delphine, Payet-Bornet, Dominique, Nozais, Mathis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698364/
https://www.ncbi.nlm.nih.gov/pubmed/36475567
http://dx.doi.org/10.1016/j.xpro.2021.101041
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author Pankaew, Saran
Grosjean, Clémence
Quessada, Julie
Loosveld, Marie
Potier, Delphine
Payet-Bornet, Dominique
Nozais, Mathis
author_facet Pankaew, Saran
Grosjean, Clémence
Quessada, Julie
Loosveld, Marie
Potier, Delphine
Payet-Bornet, Dominique
Nozais, Mathis
author_sort Pankaew, Saran
collection PubMed
description Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).
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spelling pubmed-96983642022-11-26 Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples Pankaew, Saran Grosjean, Clémence Quessada, Julie Loosveld, Marie Potier, Delphine Payet-Bornet, Dominique Nozais, Mathis STAR Protoc Protocol Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). Elsevier 2021-12-20 /pmc/articles/PMC9698364/ /pubmed/36475567 http://dx.doi.org/10.1016/j.xpro.2021.101041 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pankaew, Saran
Grosjean, Clémence
Quessada, Julie
Loosveld, Marie
Potier, Delphine
Payet-Bornet, Dominique
Nozais, Mathis
Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title_full Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title_fullStr Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title_full_unstemmed Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title_short Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
title_sort multiplexed single-cell rna-sequencing of mouse thymic and splenic samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698364/
https://www.ncbi.nlm.nih.gov/pubmed/36475567
http://dx.doi.org/10.1016/j.xpro.2021.101041
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