Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples

Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages...

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Detalles Bibliográficos
Autores principales: Pankaew, Saran, Grosjean, Clémence, Quessada, Julie, Loosveld, Marie, Potier, Delphine, Payet-Bornet, Dominique, Nozais, Mathis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698364/
https://www.ncbi.nlm.nih.gov/pubmed/36475567
http://dx.doi.org/10.1016/j.xpro.2021.101041

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