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Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis

Background: Candidaemia and invasive candidiasis are typically hospital-acquired. Genotyping isolates from patients admitted to different hospitals may be helpful in tracking clones spreading across hospitals, especially those showing antifungal resistance. Methods: We characterized Candida clusters...

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Autores principales: Díaz-García, Judith, Gómez, Ana, Machado, Marina, Alcalá, Luis, Reigadas, Elena, Sánchez-Carrillo, Carlos, Pérez-Ayala, Ana, de la Pedrosa, Elia Gómez-García, González-Romo, Fernando, Cuétara, María Soledad, García-Esteban, Coral, Quiles-Melero, Inmaculada, Zurita, Nelly Daniela, Algarra, María Muñoz, Durán-Valle, María Teresa, Sánchez-García, Aída, Muñoz, Patricia, Escribano, Pilar, Guinea, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698610/
https://www.ncbi.nlm.nih.gov/pubmed/36422050
http://dx.doi.org/10.3390/jof8111228
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author Díaz-García, Judith
Gómez, Ana
Machado, Marina
Alcalá, Luis
Reigadas, Elena
Sánchez-Carrillo, Carlos
Pérez-Ayala, Ana
de la Pedrosa, Elia Gómez-García
González-Romo, Fernando
Cuétara, María Soledad
García-Esteban, Coral
Quiles-Melero, Inmaculada
Zurita, Nelly Daniela
Algarra, María Muñoz
Durán-Valle, María Teresa
Sánchez-García, Aída
Muñoz, Patricia
Escribano, Pilar
Guinea, Jesús
author_facet Díaz-García, Judith
Gómez, Ana
Machado, Marina
Alcalá, Luis
Reigadas, Elena
Sánchez-Carrillo, Carlos
Pérez-Ayala, Ana
de la Pedrosa, Elia Gómez-García
González-Romo, Fernando
Cuétara, María Soledad
García-Esteban, Coral
Quiles-Melero, Inmaculada
Zurita, Nelly Daniela
Algarra, María Muñoz
Durán-Valle, María Teresa
Sánchez-García, Aída
Muñoz, Patricia
Escribano, Pilar
Guinea, Jesús
author_sort Díaz-García, Judith
collection PubMed
description Background: Candidaemia and invasive candidiasis are typically hospital-acquired. Genotyping isolates from patients admitted to different hospitals may be helpful in tracking clones spreading across hospitals, especially those showing antifungal resistance. Methods: We characterized Candida clusters by studying Candida isolates (C. albicans, n = 1041; C. parapsilosis, n = 354, and C. tropicalis, n = 125) from blood cultures (53.8%) and intra-abdominal samples (46.2%) collected as part of the CANDIMAD (Candida in Madrid) study in Madrid (2019–2021). Species-specific microsatellite markers were used to define the genotypes of Candida spp. found in a single patient (singleton) or several patients (cluster) from a single hospital (intra-hospital cluster) or different hospitals (widespread cluster). Results: We found 83 clusters, of which 20 were intra-hospital, 49 were widespread, and 14 were intra-hospital and widespread. Some intra-hospital clusters were first detected before the onset of the COVID-19 pandemic, but the number of clusters increased during the pandemic, especially for C. parapsilosis. The proportion of widespread clusters was significantly higher for genotypes found in both compartments than those exclusively found in either the blood cultures or intra-abdominal samples. Most C. albicans- and C. tropicalis-resistant genotypes were singleton and presented exclusively in either blood cultures or intra-abdominal samples. Fluconazole-resistant C. parapsilosis isolates belonged to intra-hospital clusters harboring either the Y132F or G458S ERG11p substitutions; the dominant genotype was also widespread. Conclusions: the number of clusters—and patients involved—increased during the COVID-19 pandemic mainly due to the emergence of fluconazole-resistant C. parapsilosis genotypes.
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spelling pubmed-96986102022-11-26 Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis Díaz-García, Judith Gómez, Ana Machado, Marina Alcalá, Luis Reigadas, Elena Sánchez-Carrillo, Carlos Pérez-Ayala, Ana de la Pedrosa, Elia Gómez-García González-Romo, Fernando Cuétara, María Soledad García-Esteban, Coral Quiles-Melero, Inmaculada Zurita, Nelly Daniela Algarra, María Muñoz Durán-Valle, María Teresa Sánchez-García, Aída Muñoz, Patricia Escribano, Pilar Guinea, Jesús J Fungi (Basel) Article Background: Candidaemia and invasive candidiasis are typically hospital-acquired. Genotyping isolates from patients admitted to different hospitals may be helpful in tracking clones spreading across hospitals, especially those showing antifungal resistance. Methods: We characterized Candida clusters by studying Candida isolates (C. albicans, n = 1041; C. parapsilosis, n = 354, and C. tropicalis, n = 125) from blood cultures (53.8%) and intra-abdominal samples (46.2%) collected as part of the CANDIMAD (Candida in Madrid) study in Madrid (2019–2021). Species-specific microsatellite markers were used to define the genotypes of Candida spp. found in a single patient (singleton) or several patients (cluster) from a single hospital (intra-hospital cluster) or different hospitals (widespread cluster). Results: We found 83 clusters, of which 20 were intra-hospital, 49 were widespread, and 14 were intra-hospital and widespread. Some intra-hospital clusters were first detected before the onset of the COVID-19 pandemic, but the number of clusters increased during the pandemic, especially for C. parapsilosis. The proportion of widespread clusters was significantly higher for genotypes found in both compartments than those exclusively found in either the blood cultures or intra-abdominal samples. Most C. albicans- and C. tropicalis-resistant genotypes were singleton and presented exclusively in either blood cultures or intra-abdominal samples. Fluconazole-resistant C. parapsilosis isolates belonged to intra-hospital clusters harboring either the Y132F or G458S ERG11p substitutions; the dominant genotype was also widespread. Conclusions: the number of clusters—and patients involved—increased during the COVID-19 pandemic mainly due to the emergence of fluconazole-resistant C. parapsilosis genotypes. MDPI 2022-11-21 /pmc/articles/PMC9698610/ /pubmed/36422050 http://dx.doi.org/10.3390/jof8111228 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Díaz-García, Judith
Gómez, Ana
Machado, Marina
Alcalá, Luis
Reigadas, Elena
Sánchez-Carrillo, Carlos
Pérez-Ayala, Ana
de la Pedrosa, Elia Gómez-García
González-Romo, Fernando
Cuétara, María Soledad
García-Esteban, Coral
Quiles-Melero, Inmaculada
Zurita, Nelly Daniela
Algarra, María Muñoz
Durán-Valle, María Teresa
Sánchez-García, Aída
Muñoz, Patricia
Escribano, Pilar
Guinea, Jesús
Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title_full Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title_fullStr Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title_full_unstemmed Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title_short Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis
title_sort candida genotyping of blood culture isolates from patients admitted to 16 hospitals in madrid: genotype spreading during the covid-19 pandemic driven by fluconazole-resistant c. parapsilosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698610/
https://www.ncbi.nlm.nih.gov/pubmed/36422050
http://dx.doi.org/10.3390/jof8111228
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