Cargando…
Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation
Naïve CD8(+) T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such ‘cytokine priming’ can promote the activation of potentially autoreactive and antitumor CD8(+) T cells by weak tissue antigens and tumor antigens. To elucida...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698886/ https://www.ncbi.nlm.nih.gov/pubmed/36430600 http://dx.doi.org/10.3390/ijms232214122 |
_version_ | 1784838931938803712 |
---|---|
author | Quenum, Akouavi Julite Irmine Santharam, Madanraj Appiya Ramanathan, Sheela Ilangumaran, Subburaj |
author_facet | Quenum, Akouavi Julite Irmine Santharam, Madanraj Appiya Ramanathan, Sheela Ilangumaran, Subburaj |
author_sort | Quenum, Akouavi Julite Irmine |
collection | PubMed |
description | Naïve CD8(+) T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such ‘cytokine priming’ can promote the activation of potentially autoreactive and antitumor CD8(+) T cells by weak tissue antigens and tumor antigens. To elucidate the molecular mechanisms of cytokine priming, naïve PMEL-1 TCR transgenic CD8(+) T lymphocytes were stimulated with IL-15 and IL-21, and chromatin accessibility was assessed using the assay for transposase-accessible chromatin (ATAC) sequencing. PMEL-1 cells stimulated by the cognate antigenic peptide mgp100(25-33) served as controls. Cytokine-primed cells showed a limited number of opening and closing chromatin accessibility peaks compared to antigen-stimulated cells. However, the ATACseq peaks in cytokine-primed cells substantially overlapped with those of antigen-stimulated cells and mapped to several genes implicated in T cell signaling, activation, effector differentiation, negative regulation and exhaustion. Nonetheless, the expression of most of these genes was remarkably different between cytokine-primed and antigen-stimulated cells. In addition, cytokine priming impacted the expression of several genes following antigen stimulation in a synergistic or antagonistic manner. Our findings indicate that chromatin accessibility changes in cytokine-primed naïve CD8(+) T cells not only underlie their increased antigen responsiveness but may also enhance their functional fitness by reducing exhaustion without compromising regulatory controls. |
format | Online Article Text |
id | pubmed-9698886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96988862022-11-26 Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation Quenum, Akouavi Julite Irmine Santharam, Madanraj Appiya Ramanathan, Sheela Ilangumaran, Subburaj Int J Mol Sci Article Naïve CD8(+) T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such ‘cytokine priming’ can promote the activation of potentially autoreactive and antitumor CD8(+) T cells by weak tissue antigens and tumor antigens. To elucidate the molecular mechanisms of cytokine priming, naïve PMEL-1 TCR transgenic CD8(+) T lymphocytes were stimulated with IL-15 and IL-21, and chromatin accessibility was assessed using the assay for transposase-accessible chromatin (ATAC) sequencing. PMEL-1 cells stimulated by the cognate antigenic peptide mgp100(25-33) served as controls. Cytokine-primed cells showed a limited number of opening and closing chromatin accessibility peaks compared to antigen-stimulated cells. However, the ATACseq peaks in cytokine-primed cells substantially overlapped with those of antigen-stimulated cells and mapped to several genes implicated in T cell signaling, activation, effector differentiation, negative regulation and exhaustion. Nonetheless, the expression of most of these genes was remarkably different between cytokine-primed and antigen-stimulated cells. In addition, cytokine priming impacted the expression of several genes following antigen stimulation in a synergistic or antagonistic manner. Our findings indicate that chromatin accessibility changes in cytokine-primed naïve CD8(+) T cells not only underlie their increased antigen responsiveness but may also enhance their functional fitness by reducing exhaustion without compromising regulatory controls. MDPI 2022-11-16 /pmc/articles/PMC9698886/ /pubmed/36430600 http://dx.doi.org/10.3390/ijms232214122 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Quenum, Akouavi Julite Irmine Santharam, Madanraj Appiya Ramanathan, Sheela Ilangumaran, Subburaj Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title | Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title_full | Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title_fullStr | Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title_full_unstemmed | Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title_short | Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8(+) T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation |
title_sort | inflammatory cytokines that enhance antigen responsiveness of naïve cd8(+) t lymphocytes modulate chromatin accessibility of genes impacted by antigen stimulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698886/ https://www.ncbi.nlm.nih.gov/pubmed/36430600 http://dx.doi.org/10.3390/ijms232214122 |
work_keys_str_mv | AT quenumakouavijuliteirmine inflammatorycytokinesthatenhanceantigenresponsivenessofnaivecd8tlymphocytesmodulatechromatinaccessibilityofgenesimpactedbyantigenstimulation AT santharammadanrajappiya inflammatorycytokinesthatenhanceantigenresponsivenessofnaivecd8tlymphocytesmodulatechromatinaccessibilityofgenesimpactedbyantigenstimulation AT ramanathansheela inflammatorycytokinesthatenhanceantigenresponsivenessofnaivecd8tlymphocytesmodulatechromatinaccessibilityofgenesimpactedbyantigenstimulation AT ilangumaransubburaj inflammatorycytokinesthatenhanceantigenresponsivenessofnaivecd8tlymphocytesmodulatechromatinaccessibilityofgenesimpactedbyantigenstimulation |