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Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes

Thymoquinone has been proved to be effective against neoplasms, including skin cancer. Its high lipophilicity, however, may limit its potential use as a drug. Melanoma remains the deadliest of all skin cancers worldwide, due to its high heterogeneity, depending on the stage of the disease. Our goal...

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Autores principales: Kłos, Patrycja, Perużyńska, Magdalena, Baśkiewicz-Hałasa, Magdalena, Skupin-Mrugalska, Paulina, Majcher, Małgorzata, Sawczuk, Magdalena, Szostak, Bartosz, Droździk, Marek, Machaliński, Bogusław, Chlubek, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698994/
https://www.ncbi.nlm.nih.gov/pubmed/36365127
http://dx.doi.org/10.3390/pharmaceutics14112309
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author Kłos, Patrycja
Perużyńska, Magdalena
Baśkiewicz-Hałasa, Magdalena
Skupin-Mrugalska, Paulina
Majcher, Małgorzata
Sawczuk, Magdalena
Szostak, Bartosz
Droździk, Marek
Machaliński, Bogusław
Chlubek, Dariusz
author_facet Kłos, Patrycja
Perużyńska, Magdalena
Baśkiewicz-Hałasa, Magdalena
Skupin-Mrugalska, Paulina
Majcher, Małgorzata
Sawczuk, Magdalena
Szostak, Bartosz
Droździk, Marek
Machaliński, Bogusław
Chlubek, Dariusz
author_sort Kłos, Patrycja
collection PubMed
description Thymoquinone has been proved to be effective against neoplasms, including skin cancer. Its high lipophilicity, however, may limit its potential use as a drug. Melanoma remains the deadliest of all skin cancers worldwide, due to its high heterogeneity, depending on the stage of the disease. Our goal was to compare the anti-cancer activity of free thymoquinone and thymoquinone-loaded liposomes on two melanoma cell lines that originated from different stages of this cancer: skin-derived A375 and metastatic WM9. We evaluated the proapoptotic effects of free thymoquinone by flow cytometry and Western blot, and its mitotoxicity by means of JC-1 assay. Additionally, we compared the cytotoxicity of free thymoquinone and thymoquinone in liposomes by WST-1 assay. Our results revealed a higher antiproliferative effect of TQ in WM9 cells, whereas its higher proapoptotic activity was observed in the A375 cell line. Moreover, the thymoquinone-loaded liposome was proved to exert stronger cytotoxic effect on both cell lines studied than free thymoquinone. Differences in the response of melanoma cells derived from different stages of the disease to thymoquinone, as well as their different responses to free and carrier-delivered thymoquinone, are essential for the development of new anti-melanoma therapies. However, further research is required to fully understand them.
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spelling pubmed-96989942022-11-26 Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes Kłos, Patrycja Perużyńska, Magdalena Baśkiewicz-Hałasa, Magdalena Skupin-Mrugalska, Paulina Majcher, Małgorzata Sawczuk, Magdalena Szostak, Bartosz Droździk, Marek Machaliński, Bogusław Chlubek, Dariusz Pharmaceutics Article Thymoquinone has been proved to be effective against neoplasms, including skin cancer. Its high lipophilicity, however, may limit its potential use as a drug. Melanoma remains the deadliest of all skin cancers worldwide, due to its high heterogeneity, depending on the stage of the disease. Our goal was to compare the anti-cancer activity of free thymoquinone and thymoquinone-loaded liposomes on two melanoma cell lines that originated from different stages of this cancer: skin-derived A375 and metastatic WM9. We evaluated the proapoptotic effects of free thymoquinone by flow cytometry and Western blot, and its mitotoxicity by means of JC-1 assay. Additionally, we compared the cytotoxicity of free thymoquinone and thymoquinone in liposomes by WST-1 assay. Our results revealed a higher antiproliferative effect of TQ in WM9 cells, whereas its higher proapoptotic activity was observed in the A375 cell line. Moreover, the thymoquinone-loaded liposome was proved to exert stronger cytotoxic effect on both cell lines studied than free thymoquinone. Differences in the response of melanoma cells derived from different stages of the disease to thymoquinone, as well as their different responses to free and carrier-delivered thymoquinone, are essential for the development of new anti-melanoma therapies. However, further research is required to fully understand them. MDPI 2022-10-27 /pmc/articles/PMC9698994/ /pubmed/36365127 http://dx.doi.org/10.3390/pharmaceutics14112309 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kłos, Patrycja
Perużyńska, Magdalena
Baśkiewicz-Hałasa, Magdalena
Skupin-Mrugalska, Paulina
Majcher, Małgorzata
Sawczuk, Magdalena
Szostak, Bartosz
Droździk, Marek
Machaliński, Bogusław
Chlubek, Dariusz
Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title_full Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title_fullStr Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title_full_unstemmed Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title_short Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
title_sort response of skin-derived and metastatic human malignant melanoma cell lines to thymoquinone and thymoquinone-loaded liposomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698994/
https://www.ncbi.nlm.nih.gov/pubmed/36365127
http://dx.doi.org/10.3390/pharmaceutics14112309
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