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Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †

Three-dimensional chitinous scaffolds often used in regenerative medicine, tissue engineering, biomimetics and technology are mostly isolated from marine organisms, such as marine sponges (Porifera). In this work, we report the results of the electrochemical isolation of the ready to use chitinous m...

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Autores principales: Nowacki, Krzysztof, Galiński, Maciej, Fursov, Andriy, Voronkina, Alona, Meissner, Heike, Petrenko, Iaroslav, Stelling, Allison L., Ehrlich, Hermann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699038/
https://www.ncbi.nlm.nih.gov/pubmed/36354988
http://dx.doi.org/10.3390/md20110665
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author Nowacki, Krzysztof
Galiński, Maciej
Fursov, Andriy
Voronkina, Alona
Meissner, Heike
Petrenko, Iaroslav
Stelling, Allison L.
Ehrlich, Hermann
author_facet Nowacki, Krzysztof
Galiński, Maciej
Fursov, Andriy
Voronkina, Alona
Meissner, Heike
Petrenko, Iaroslav
Stelling, Allison L.
Ehrlich, Hermann
author_sort Nowacki, Krzysztof
collection PubMed
description Three-dimensional chitinous scaffolds often used in regenerative medicine, tissue engineering, biomimetics and technology are mostly isolated from marine organisms, such as marine sponges (Porifera). In this work, we report the results of the electrochemical isolation of the ready to use chitinous matrices from three species of verongiid demosponges (Aplysina archeri, Ianthella basta and Suberea clavata) as a perfect example of possible morphological and chemical dimorphism in the case of the marine chitin sources. The electrolysis of concentrated Na(2)SO(4) aqueous solution showed its superiority over the chemical chitin isolation method in terms of the treatment time reduction: only 5.5 h for A. archeri, 16.5 h for I. basta and 20 h for the S. clavata sample. Further investigation of the isolated scaffolds by digital microscopy and SEM showed that the electrolysis-supported isolation process obtains chitinous scaffolds with well-preserved spatial structure and it can be competitive to other alternative chitin isolation techniques that use external accelerating factors such as microwave irradiation or atmospheric plasma. Moreover, the infrared spectroscopy (ATR-FTIR) proved that with the applied electrochemical conditions, the transformation into chitosan does not take place.
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spelling pubmed-96990382022-11-26 Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges † Nowacki, Krzysztof Galiński, Maciej Fursov, Andriy Voronkina, Alona Meissner, Heike Petrenko, Iaroslav Stelling, Allison L. Ehrlich, Hermann Mar Drugs Article Three-dimensional chitinous scaffolds often used in regenerative medicine, tissue engineering, biomimetics and technology are mostly isolated from marine organisms, such as marine sponges (Porifera). In this work, we report the results of the electrochemical isolation of the ready to use chitinous matrices from three species of verongiid demosponges (Aplysina archeri, Ianthella basta and Suberea clavata) as a perfect example of possible morphological and chemical dimorphism in the case of the marine chitin sources. The electrolysis of concentrated Na(2)SO(4) aqueous solution showed its superiority over the chemical chitin isolation method in terms of the treatment time reduction: only 5.5 h for A. archeri, 16.5 h for I. basta and 20 h for the S. clavata sample. Further investigation of the isolated scaffolds by digital microscopy and SEM showed that the electrolysis-supported isolation process obtains chitinous scaffolds with well-preserved spatial structure and it can be competitive to other alternative chitin isolation techniques that use external accelerating factors such as microwave irradiation or atmospheric plasma. Moreover, the infrared spectroscopy (ATR-FTIR) proved that with the applied electrochemical conditions, the transformation into chitosan does not take place. MDPI 2022-10-25 /pmc/articles/PMC9699038/ /pubmed/36354988 http://dx.doi.org/10.3390/md20110665 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nowacki, Krzysztof
Galiński, Maciej
Fursov, Andriy
Voronkina, Alona
Meissner, Heike
Petrenko, Iaroslav
Stelling, Allison L.
Ehrlich, Hermann
Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title_full Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title_fullStr Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title_full_unstemmed Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title_short Electrolysis as a Universal Approach for Isolation of Diverse Chitin Scaffolds from Selected Marine Demosponges †
title_sort electrolysis as a universal approach for isolation of diverse chitin scaffolds from selected marine demosponges †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699038/
https://www.ncbi.nlm.nih.gov/pubmed/36354988
http://dx.doi.org/10.3390/md20110665
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