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Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland

Escherichia albertii, a zoonotic pathogen, has sporadically been associated with infectious diarrhea in humans. Poultry and wild birds are considered potential reservoirs. We assessed the occurrence of E. albertii in 280 fecal samples from wild birds (n = 130) and pooled fecal samples collected at s...

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Autores principales: Barmettler, Karen, Biggel, Michael, Treier, Andrea, Muchaamba, Francis, Vogler, Barbara Renate, Stephan, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699108/
https://www.ncbi.nlm.nih.gov/pubmed/36422334
http://dx.doi.org/10.3390/microorganisms10112265
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author Barmettler, Karen
Biggel, Michael
Treier, Andrea
Muchaamba, Francis
Vogler, Barbara Renate
Stephan, Roger
author_facet Barmettler, Karen
Biggel, Michael
Treier, Andrea
Muchaamba, Francis
Vogler, Barbara Renate
Stephan, Roger
author_sort Barmettler, Karen
collection PubMed
description Escherichia albertii, a zoonotic pathogen, has sporadically been associated with infectious diarrhea in humans. Poultry and wild birds are considered potential reservoirs. We assessed the occurrence of E. albertii in 280 fecal samples from wild birds (n = 130) and pooled fecal samples collected at slaughterhouse level from poultry flocks (n = 150) in Switzerland. Using an E. albertii-specific PCR targeting the Eacdt gene, 23.8% (31/130) of the samples from wild birds, but not from the pooled poultry fecal samples, tested positive for Eacdt. The positive samples originated from 11 bird species belonging to eight families. Strain isolation was attempted on the PCR-positive samples by subculturing the broth cultures onto xylose–MacConkey plates. Isolation was possible on 12 of the 31 Eacdt-PCR-positive samples. Whole-genome sequencing revealed that the strains belonged to nine distinct sequence types, with ST13420 and ST5967 being represented by two and three isolates, respectively. All strains harbored the eae gene, while two strains were also positive for stx2f. Our study thus shows that E. albertii is present in the Swiss wild bird population, which can potentially act as a source of this pathogen to humans, other animals, and the environment.
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spelling pubmed-96991082022-11-26 Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland Barmettler, Karen Biggel, Michael Treier, Andrea Muchaamba, Francis Vogler, Barbara Renate Stephan, Roger Microorganisms Article Escherichia albertii, a zoonotic pathogen, has sporadically been associated with infectious diarrhea in humans. Poultry and wild birds are considered potential reservoirs. We assessed the occurrence of E. albertii in 280 fecal samples from wild birds (n = 130) and pooled fecal samples collected at slaughterhouse level from poultry flocks (n = 150) in Switzerland. Using an E. albertii-specific PCR targeting the Eacdt gene, 23.8% (31/130) of the samples from wild birds, but not from the pooled poultry fecal samples, tested positive for Eacdt. The positive samples originated from 11 bird species belonging to eight families. Strain isolation was attempted on the PCR-positive samples by subculturing the broth cultures onto xylose–MacConkey plates. Isolation was possible on 12 of the 31 Eacdt-PCR-positive samples. Whole-genome sequencing revealed that the strains belonged to nine distinct sequence types, with ST13420 and ST5967 being represented by two and three isolates, respectively. All strains harbored the eae gene, while two strains were also positive for stx2f. Our study thus shows that E. albertii is present in the Swiss wild bird population, which can potentially act as a source of this pathogen to humans, other animals, and the environment. MDPI 2022-11-15 /pmc/articles/PMC9699108/ /pubmed/36422334 http://dx.doi.org/10.3390/microorganisms10112265 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barmettler, Karen
Biggel, Michael
Treier, Andrea
Muchaamba, Francis
Vogler, Barbara Renate
Stephan, Roger
Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title_full Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title_fullStr Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title_full_unstemmed Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title_short Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
title_sort occurrence and characteristics of escherichia albertii in wild birds and poultry flocks in switzerland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699108/
https://www.ncbi.nlm.nih.gov/pubmed/36422334
http://dx.doi.org/10.3390/microorganisms10112265
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