Cargando…

Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection

Hematopoietic stem and progenitor cells (HSPCs) mobilization is the movement of HSPCs from the bone marrow to the peripheral blood or tissue induced by stress. HSPC mobilization is a well-known response to protect the host during infection through urgent differentiation of HSPCs to immune cells. Den...

Descripción completa

Detalles Bibliográficos
Autores principales: Puc, Irwin, Ho, Tzu-Chuan, Chien, Yu-Wen, Tan, Sia-Seng, Fong, Yu-Cin, Chen, Yi-Ju, Wang, Sheng-Hsuan, Li, Yun-Hsuan, Chen, Chun-Hong, Chen, Po-Lin, Perng, Guey-Chuen, Tsai, Jih-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699116/
https://www.ncbi.nlm.nih.gov/pubmed/36430807
http://dx.doi.org/10.3390/ijms232214330
_version_ 1784838991970828288
author Puc, Irwin
Ho, Tzu-Chuan
Chien, Yu-Wen
Tan, Sia-Seng
Fong, Yu-Cin
Chen, Yi-Ju
Wang, Sheng-Hsuan
Li, Yun-Hsuan
Chen, Chun-Hong
Chen, Po-Lin
Perng, Guey-Chuen
Tsai, Jih-Jin
author_facet Puc, Irwin
Ho, Tzu-Chuan
Chien, Yu-Wen
Tan, Sia-Seng
Fong, Yu-Cin
Chen, Yi-Ju
Wang, Sheng-Hsuan
Li, Yun-Hsuan
Chen, Chun-Hong
Chen, Po-Lin
Perng, Guey-Chuen
Tsai, Jih-Jin
author_sort Puc, Irwin
collection PubMed
description Hematopoietic stem and progenitor cells (HSPCs) mobilization is the movement of HSPCs from the bone marrow to the peripheral blood or tissue induced by stress. HSPC mobilization is a well-known response to protect the host during infection through urgent differentiation of HSPCs to immune cells. Dengue virus (DENV) infection is known to cause stress in infected humans and the mobilizing capacity of HSPCs during DENV infection in affected patients has not been fully investigated. Here, we investigated whether DENV infection can induce HSPC mobilization and if the mobilized HSPCs are permissive to DENV infection. White blood cells (WBCs) were collected from dengue patients (DENV+) and healthy donors and analyzed by flow cytometry and plaque assay. Elevated HSPCs levels were found in the WBCs of the DENV+ group when compared to the healthy group. Mobilization of HSPCs and homing markers (skin and gut) expression decreased as the patients proceeded from dengue without symptoms (DWoWS) to severe dengue (SD). Mobilizing HSPCs were not only permissive to DENV infection, but infectious DENV could be recovered after coculture. Our results highlight the need for further investigation into HSPC mobilization or alterations of hematopoiesis during viral infections such as DENV in order to develop appropriate countermeasures.
format Online
Article
Text
id pubmed-9699116
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96991162022-11-26 Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection Puc, Irwin Ho, Tzu-Chuan Chien, Yu-Wen Tan, Sia-Seng Fong, Yu-Cin Chen, Yi-Ju Wang, Sheng-Hsuan Li, Yun-Hsuan Chen, Chun-Hong Chen, Po-Lin Perng, Guey-Chuen Tsai, Jih-Jin Int J Mol Sci Article Hematopoietic stem and progenitor cells (HSPCs) mobilization is the movement of HSPCs from the bone marrow to the peripheral blood or tissue induced by stress. HSPC mobilization is a well-known response to protect the host during infection through urgent differentiation of HSPCs to immune cells. Dengue virus (DENV) infection is known to cause stress in infected humans and the mobilizing capacity of HSPCs during DENV infection in affected patients has not been fully investigated. Here, we investigated whether DENV infection can induce HSPC mobilization and if the mobilized HSPCs are permissive to DENV infection. White blood cells (WBCs) were collected from dengue patients (DENV+) and healthy donors and analyzed by flow cytometry and plaque assay. Elevated HSPCs levels were found in the WBCs of the DENV+ group when compared to the healthy group. Mobilization of HSPCs and homing markers (skin and gut) expression decreased as the patients proceeded from dengue without symptoms (DWoWS) to severe dengue (SD). Mobilizing HSPCs were not only permissive to DENV infection, but infectious DENV could be recovered after coculture. Our results highlight the need for further investigation into HSPC mobilization or alterations of hematopoiesis during viral infections such as DENV in order to develop appropriate countermeasures. MDPI 2022-11-18 /pmc/articles/PMC9699116/ /pubmed/36430807 http://dx.doi.org/10.3390/ijms232214330 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Puc, Irwin
Ho, Tzu-Chuan
Chien, Yu-Wen
Tan, Sia-Seng
Fong, Yu-Cin
Chen, Yi-Ju
Wang, Sheng-Hsuan
Li, Yun-Hsuan
Chen, Chun-Hong
Chen, Po-Lin
Perng, Guey-Chuen
Tsai, Jih-Jin
Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title_full Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title_fullStr Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title_full_unstemmed Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title_short Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
title_sort mobilization of hematopoietic stem and progenitor cells during dengue virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699116/
https://www.ncbi.nlm.nih.gov/pubmed/36430807
http://dx.doi.org/10.3390/ijms232214330
work_keys_str_mv AT pucirwin mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT hotzuchuan mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT chienyuwen mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT tansiaseng mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT fongyucin mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT chenyiju mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT wangshenghsuan mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT liyunhsuan mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT chenchunhong mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT chenpolin mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT pernggueychuen mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection
AT tsaijihjin mobilizationofhematopoieticstemandprogenitorcellsduringdenguevirusinfection