Cargando…

Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard

Brassica carinata (BBCC, 2n = 34) is commonly known as Ethiopian mustard, Abyssinian mustard, or carinata. Its excellent agronomic traits, including resistance to biotic and abiotic stresses, make it a potential genetic donor for interspecific hybridization. Myzus persicae (green peach aphid, GPA) i...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Fangyuan, Chen, Chaoquan, Kong, Lijun, Liu, Shenglanjia, Zhao, Kun, Zhang, Yi, Zhao, Tong, Liu, Kaiwen, Yu, Xiaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699141/
https://www.ncbi.nlm.nih.gov/pubmed/36430212
http://dx.doi.org/10.3390/ijms232213736
_version_ 1784838998425862144
author Zhou, Fangyuan
Chen, Chaoquan
Kong, Lijun
Liu, Shenglanjia
Zhao, Kun
Zhang, Yi
Zhao, Tong
Liu, Kaiwen
Yu, Xiaolin
author_facet Zhou, Fangyuan
Chen, Chaoquan
Kong, Lijun
Liu, Shenglanjia
Zhao, Kun
Zhang, Yi
Zhao, Tong
Liu, Kaiwen
Yu, Xiaolin
author_sort Zhou, Fangyuan
collection PubMed
description Brassica carinata (BBCC, 2n = 34) is commonly known as Ethiopian mustard, Abyssinian mustard, or carinata. Its excellent agronomic traits, including resistance to biotic and abiotic stresses, make it a potential genetic donor for interspecific hybridization. Myzus persicae (green peach aphid, GPA) is one of the most harmful pests of Brassica crops, significantly effecting the yield and quality. However, few aphid-resistant Brassica crop germplasms have been utilized in breeding practices, while the underlying biochemical basis of aphid resistance still remains poorly understood. In this study, we examined the genetic diversity of 75 B. carinata accessions and some plant characteristics that potentially contribute to GPA resistance. Initially, the morphological characterization showed abundant diversity in the phenotypic traits, with the dendrogram indicating that the genetic variation of the 75 accessions ranged from 0.66 to 0.98. A population structure analysis revealed that these accessions could be grouped into two main subpopulations and one admixed group, with the majority of accessions (86.67%) clustering in one subpopulation. Subsequently, there were three GPA-resistant B. carinata accessions, BC13, BC47, and BC51. The electrical penetration graph (EPG) assay detected resistance factors in the leaf mesophyll tissue and xylem. The result demonstrated that the Ethiopian mustard accessions were susceptible when the phloem probing time, the first probe time, and the G-wave time were 20.51–32.51 min, 26.36–55.54 s, and 36.18–47.84 min, respectively. In contrast, resistance of the Ethiopian mustard accessions was observed with the phloem probing time, the first probe time, and G-wave time of 41.18–70.78 min, 181.07–365.85 s, and 18.03–26.37 min, respectively. In addition, the epidermal characters, leaf anatomical structure, glucosinolate composition, defense-related enzyme activities, and callose deposition were compared between the resistant and susceptible accessions. GPA-resistant accessions had denser longitudinal leaf structure, higher wax content on the leaf surface, higher indole glucosinolate level, increased polyphenol oxidase (PPO) activity, and faster callose deposition than the susceptible accessions. This study validates that inherent physical and chemical barriers are evidently crucial factors in the resistance against GPA infestation. This study not only provide new insights into the biochemical basis of GPA resistance but also highlights the GPA-resistant B. carinata germplasm resources for the future accurate genetic improvement of Brassica crops.
format Online
Article
Text
id pubmed-9699141
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96991412022-11-26 Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard Zhou, Fangyuan Chen, Chaoquan Kong, Lijun Liu, Shenglanjia Zhao, Kun Zhang, Yi Zhao, Tong Liu, Kaiwen Yu, Xiaolin Int J Mol Sci Article Brassica carinata (BBCC, 2n = 34) is commonly known as Ethiopian mustard, Abyssinian mustard, or carinata. Its excellent agronomic traits, including resistance to biotic and abiotic stresses, make it a potential genetic donor for interspecific hybridization. Myzus persicae (green peach aphid, GPA) is one of the most harmful pests of Brassica crops, significantly effecting the yield and quality. However, few aphid-resistant Brassica crop germplasms have been utilized in breeding practices, while the underlying biochemical basis of aphid resistance still remains poorly understood. In this study, we examined the genetic diversity of 75 B. carinata accessions and some plant characteristics that potentially contribute to GPA resistance. Initially, the morphological characterization showed abundant diversity in the phenotypic traits, with the dendrogram indicating that the genetic variation of the 75 accessions ranged from 0.66 to 0.98. A population structure analysis revealed that these accessions could be grouped into two main subpopulations and one admixed group, with the majority of accessions (86.67%) clustering in one subpopulation. Subsequently, there were three GPA-resistant B. carinata accessions, BC13, BC47, and BC51. The electrical penetration graph (EPG) assay detected resistance factors in the leaf mesophyll tissue and xylem. The result demonstrated that the Ethiopian mustard accessions were susceptible when the phloem probing time, the first probe time, and the G-wave time were 20.51–32.51 min, 26.36–55.54 s, and 36.18–47.84 min, respectively. In contrast, resistance of the Ethiopian mustard accessions was observed with the phloem probing time, the first probe time, and G-wave time of 41.18–70.78 min, 181.07–365.85 s, and 18.03–26.37 min, respectively. In addition, the epidermal characters, leaf anatomical structure, glucosinolate composition, defense-related enzyme activities, and callose deposition were compared between the resistant and susceptible accessions. GPA-resistant accessions had denser longitudinal leaf structure, higher wax content on the leaf surface, higher indole glucosinolate level, increased polyphenol oxidase (PPO) activity, and faster callose deposition than the susceptible accessions. This study validates that inherent physical and chemical barriers are evidently crucial factors in the resistance against GPA infestation. This study not only provide new insights into the biochemical basis of GPA resistance but also highlights the GPA-resistant B. carinata germplasm resources for the future accurate genetic improvement of Brassica crops. MDPI 2022-11-08 /pmc/articles/PMC9699141/ /pubmed/36430212 http://dx.doi.org/10.3390/ijms232213736 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Fangyuan
Chen, Chaoquan
Kong, Lijun
Liu, Shenglanjia
Zhao, Kun
Zhang, Yi
Zhao, Tong
Liu, Kaiwen
Yu, Xiaolin
Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title_full Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title_fullStr Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title_full_unstemmed Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title_short Genetic Diversity Analysis Reveals Potential of the Green Peach Aphid (Myzus persicae) Resistance in Ethiopian Mustard
title_sort genetic diversity analysis reveals potential of the green peach aphid (myzus persicae) resistance in ethiopian mustard
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699141/
https://www.ncbi.nlm.nih.gov/pubmed/36430212
http://dx.doi.org/10.3390/ijms232213736
work_keys_str_mv AT zhoufangyuan geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT chenchaoquan geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT konglijun geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT liushenglanjia geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT zhaokun geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT zhangyi geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT zhaotong geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT liukaiwen geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard
AT yuxiaolin geneticdiversityanalysisrevealspotentialofthegreenpeachaphidmyzuspersicaeresistanceinethiopianmustard