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Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract
Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699243/ https://www.ncbi.nlm.nih.gov/pubmed/36422242 http://dx.doi.org/10.3390/metabo12111102 |
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author | Zayed, Muhammad El-Garawani, Islam M. El-Sabbagh, Sabha M. Amr, Bassem Alsharif, Sultan M. Tayel, Ahmed A. AlAjmi, Mohamed F. Ibrahim, Hasnaa M. S. Shou, Qiyang Khalifa, Shaden A. M. El-Seedi, Hesham R. Elfeky, Nora |
author_facet | Zayed, Muhammad El-Garawani, Islam M. El-Sabbagh, Sabha M. Amr, Bassem Alsharif, Sultan M. Tayel, Ahmed A. AlAjmi, Mohamed F. Ibrahim, Hasnaa M. S. Shou, Qiyang Khalifa, Shaden A. M. El-Seedi, Hesham R. Elfeky, Nora |
author_sort | Zayed, Muhammad |
collection | PubMed |
description | Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial strains. A bacterial isolate (BF202) cultured from the marine sediments of Alexandria’s Mariout Lake (Egypt) was tested for its antimicrobial and anticancer potential. The phylogenetic analysis of the isolated strain’s 16S rDNA and gyrB revealed that BF202 belongs to Brevibacillus laterosporus (B. laterosporus). Antibiosis of B. laterosporus was confirmed against microbial pathogens including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Staphylococcus aureus. The highest antibacterial activity was detected on glucose peptone medium after 18 h of incubation at 35 °C, and at pH of 7.0 in the presence of mannose and ammonium carbonate as carbon and nitrogen sources, respectively. The cytotoxicity of the methanolic extract against breast cancer (MCF-7) and normal Vero cell lines, using the MTT test, revealed IC(50) values of 7.93 and 23.79 µg/mL, respectively. To identify apoptotic and necrotic cells, a flow cytometric analysis using annexin V-FITC/PI dual-labeling was utilized and recorded a higher number of necrotic cells compared to apoptotic ones. Similarly, the cell cycle S-phase arrest was reported. The LC-MS-MS investigation of B. laterosporus extract and the molecular networking database analysis demonstrated five strategic diketopiperazine compounds with antimicrobial and anticancer activities. Taken together, this research shows that the crude extract of B. laterosporus might be an effective agent against drug-resistant bacteria and malignant disorders due to its richness in diketopiperazines. |
format | Online Article Text |
id | pubmed-9699243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96992432022-11-26 Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract Zayed, Muhammad El-Garawani, Islam M. El-Sabbagh, Sabha M. Amr, Bassem Alsharif, Sultan M. Tayel, Ahmed A. AlAjmi, Mohamed F. Ibrahim, Hasnaa M. S. Shou, Qiyang Khalifa, Shaden A. M. El-Seedi, Hesham R. Elfeky, Nora Metabolites Article Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial strains. A bacterial isolate (BF202) cultured from the marine sediments of Alexandria’s Mariout Lake (Egypt) was tested for its antimicrobial and anticancer potential. The phylogenetic analysis of the isolated strain’s 16S rDNA and gyrB revealed that BF202 belongs to Brevibacillus laterosporus (B. laterosporus). Antibiosis of B. laterosporus was confirmed against microbial pathogens including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Staphylococcus aureus. The highest antibacterial activity was detected on glucose peptone medium after 18 h of incubation at 35 °C, and at pH of 7.0 in the presence of mannose and ammonium carbonate as carbon and nitrogen sources, respectively. The cytotoxicity of the methanolic extract against breast cancer (MCF-7) and normal Vero cell lines, using the MTT test, revealed IC(50) values of 7.93 and 23.79 µg/mL, respectively. To identify apoptotic and necrotic cells, a flow cytometric analysis using annexin V-FITC/PI dual-labeling was utilized and recorded a higher number of necrotic cells compared to apoptotic ones. Similarly, the cell cycle S-phase arrest was reported. The LC-MS-MS investigation of B. laterosporus extract and the molecular networking database analysis demonstrated five strategic diketopiperazine compounds with antimicrobial and anticancer activities. Taken together, this research shows that the crude extract of B. laterosporus might be an effective agent against drug-resistant bacteria and malignant disorders due to its richness in diketopiperazines. MDPI 2022-11-11 /pmc/articles/PMC9699243/ /pubmed/36422242 http://dx.doi.org/10.3390/metabo12111102 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zayed, Muhammad El-Garawani, Islam M. El-Sabbagh, Sabha M. Amr, Bassem Alsharif, Sultan M. Tayel, Ahmed A. AlAjmi, Mohamed F. Ibrahim, Hasnaa M. S. Shou, Qiyang Khalifa, Shaden A. M. El-Seedi, Hesham R. Elfeky, Nora Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title | Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title_full | Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title_fullStr | Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title_full_unstemmed | Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title_short | Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract |
title_sort | structural diversity, lc-ms-ms analysis and potential biological activities of brevibacillus laterosporus extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699243/ https://www.ncbi.nlm.nih.gov/pubmed/36422242 http://dx.doi.org/10.3390/metabo12111102 |
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