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Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications

There is an urgent need for predictive in vitro models to improve disease modeling and drug target identification and validation, especially for neurological disorders. Cerebral organoids, as alternative methods to in vivo studies, appear now as powerful tools to decipher complex biological processe...

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Autores principales: Castiglione, Héloïse, Vigneron, Pierre-Antoine, Baquerre, Camille, Yates, Frank, Rontard, Jessica, Honegger, Thibault
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699341/
https://www.ncbi.nlm.nih.gov/pubmed/36365119
http://dx.doi.org/10.3390/pharmaceutics14112301
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author Castiglione, Héloïse
Vigneron, Pierre-Antoine
Baquerre, Camille
Yates, Frank
Rontard, Jessica
Honegger, Thibault
author_facet Castiglione, Héloïse
Vigneron, Pierre-Antoine
Baquerre, Camille
Yates, Frank
Rontard, Jessica
Honegger, Thibault
author_sort Castiglione, Héloïse
collection PubMed
description There is an urgent need for predictive in vitro models to improve disease modeling and drug target identification and validation, especially for neurological disorders. Cerebral organoids, as alternative methods to in vivo studies, appear now as powerful tools to decipher complex biological processes thanks to their ability to recapitulate many features of the human brain. Combining these innovative models with microfluidic technologies, referred to as brain organoids-on-chips, allows us to model the microenvironment of several neuronal cell types in 3D. Thus, this platform opens new avenues to create a relevant in vitro approach for preclinical applications in neuroscience. The transfer to the pharmaceutical industry in drug discovery stages and the adoption of this approach by the scientific community requires the proposition of innovative microphysiological systems allowing the generation of reproducible cerebral organoids of high quality in terms of structural and functional maturation, and compatibility with automation processes and high-throughput screening. In this review, we will focus on the promising advantages of cerebral organoids for disease modeling and how their combination with microfluidic systems can enhance the reproducibility and quality of these in vitro models. Then, we will finish by explaining why brain organoids-on-chips could be considered promising platforms for pharmacological applications.
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spelling pubmed-96993412022-11-26 Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications Castiglione, Héloïse Vigneron, Pierre-Antoine Baquerre, Camille Yates, Frank Rontard, Jessica Honegger, Thibault Pharmaceutics Review There is an urgent need for predictive in vitro models to improve disease modeling and drug target identification and validation, especially for neurological disorders. Cerebral organoids, as alternative methods to in vivo studies, appear now as powerful tools to decipher complex biological processes thanks to their ability to recapitulate many features of the human brain. Combining these innovative models with microfluidic technologies, referred to as brain organoids-on-chips, allows us to model the microenvironment of several neuronal cell types in 3D. Thus, this platform opens new avenues to create a relevant in vitro approach for preclinical applications in neuroscience. The transfer to the pharmaceutical industry in drug discovery stages and the adoption of this approach by the scientific community requires the proposition of innovative microphysiological systems allowing the generation of reproducible cerebral organoids of high quality in terms of structural and functional maturation, and compatibility with automation processes and high-throughput screening. In this review, we will focus on the promising advantages of cerebral organoids for disease modeling and how their combination with microfluidic systems can enhance the reproducibility and quality of these in vitro models. Then, we will finish by explaining why brain organoids-on-chips could be considered promising platforms for pharmacological applications. MDPI 2022-10-26 /pmc/articles/PMC9699341/ /pubmed/36365119 http://dx.doi.org/10.3390/pharmaceutics14112301 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Castiglione, Héloïse
Vigneron, Pierre-Antoine
Baquerre, Camille
Yates, Frank
Rontard, Jessica
Honegger, Thibault
Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title_full Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title_fullStr Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title_full_unstemmed Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title_short Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications
title_sort human brain organoids-on-chip: advances, challenges, and perspectives for preclinical applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699341/
https://www.ncbi.nlm.nih.gov/pubmed/36365119
http://dx.doi.org/10.3390/pharmaceutics14112301
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