Cargando…

Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation

Catheter-associated urinary tract infections (CAUTIs) are significant complications among catheterized patients, resulting in increased morbidity, mortality rates, and healthcare costs. Foley urinary catheters coated with synthesized silver nanoparticles (AgNPs) using Eucalyptus camaldulensis leaf e...

Descripción completa

Detalles Bibliográficos
Autores principales: Lethongkam, Sakkarin, Paosen, Supakit, Bilhman, Siwaporn, Dumjun, Krittima, Wunnoo, Suttiwan, Choojit, Suntree, Siri, Ratchaneewan, Daengngam, Chalongrat, Voravuthikunchai, Supayang P., Bejrananda, Tanan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699417/
https://www.ncbi.nlm.nih.gov/pubmed/36432345
http://dx.doi.org/10.3390/nano12224059
_version_ 1784839067989442560
author Lethongkam, Sakkarin
Paosen, Supakit
Bilhman, Siwaporn
Dumjun, Krittima
Wunnoo, Suttiwan
Choojit, Suntree
Siri, Ratchaneewan
Daengngam, Chalongrat
Voravuthikunchai, Supayang P.
Bejrananda, Tanan
author_facet Lethongkam, Sakkarin
Paosen, Supakit
Bilhman, Siwaporn
Dumjun, Krittima
Wunnoo, Suttiwan
Choojit, Suntree
Siri, Ratchaneewan
Daengngam, Chalongrat
Voravuthikunchai, Supayang P.
Bejrananda, Tanan
author_sort Lethongkam, Sakkarin
collection PubMed
description Catheter-associated urinary tract infections (CAUTIs) are significant complications among catheterized patients, resulting in increased morbidity, mortality rates, and healthcare costs. Foley urinary catheters coated with synthesized silver nanoparticles (AgNPs) using Eucalyptus camaldulensis leaf extract were developed using a green chemistry principle. In situ-deposited AgNPs with particle size ranging between 20 and 120 nm on the catheter surface were illustrated by scanning electron microscopy. Atomic force microscopy revealed the changes in surface roughness after coating with nanoparticles. The coated catheter could significantly inhibit microbial adhesion and biofilm formation performed in pooled human urine-supplemented media to mimic a microenvironment during infections (p 0.05). AgNPs-coated catheter exhibited broad-spectrum antimicrobial activity against important pathogens, causing CAUTIs with no cytotoxic effects on HeLa cells. A reduction in microbial viability in biofilms was observed under confocal laser scanning microscopy. A catheter bridge model demonstrated complete prevention of Proteus mirabilis migration by the coated catheter. Significant inhibition of ascending motility of Escherichia coli and P. mirabilis along the AgNPs-coated catheter was demonstrated in an in vitro bladder model (p 0.05). The results suggested that the AgNPs-coated urinary catheter could be applied as an alternative strategy to minimize the risk of CAUTIs by preventing bacterial colonization and biofilm formation.
format Online
Article
Text
id pubmed-9699417
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96994172022-11-26 Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation Lethongkam, Sakkarin Paosen, Supakit Bilhman, Siwaporn Dumjun, Krittima Wunnoo, Suttiwan Choojit, Suntree Siri, Ratchaneewan Daengngam, Chalongrat Voravuthikunchai, Supayang P. Bejrananda, Tanan Nanomaterials (Basel) Article Catheter-associated urinary tract infections (CAUTIs) are significant complications among catheterized patients, resulting in increased morbidity, mortality rates, and healthcare costs. Foley urinary catheters coated with synthesized silver nanoparticles (AgNPs) using Eucalyptus camaldulensis leaf extract were developed using a green chemistry principle. In situ-deposited AgNPs with particle size ranging between 20 and 120 nm on the catheter surface were illustrated by scanning electron microscopy. Atomic force microscopy revealed the changes in surface roughness after coating with nanoparticles. The coated catheter could significantly inhibit microbial adhesion and biofilm formation performed in pooled human urine-supplemented media to mimic a microenvironment during infections (p 0.05). AgNPs-coated catheter exhibited broad-spectrum antimicrobial activity against important pathogens, causing CAUTIs with no cytotoxic effects on HeLa cells. A reduction in microbial viability in biofilms was observed under confocal laser scanning microscopy. A catheter bridge model demonstrated complete prevention of Proteus mirabilis migration by the coated catheter. Significant inhibition of ascending motility of Escherichia coli and P. mirabilis along the AgNPs-coated catheter was demonstrated in an in vitro bladder model (p 0.05). The results suggested that the AgNPs-coated urinary catheter could be applied as an alternative strategy to minimize the risk of CAUTIs by preventing bacterial colonization and biofilm formation. MDPI 2022-11-18 /pmc/articles/PMC9699417/ /pubmed/36432345 http://dx.doi.org/10.3390/nano12224059 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lethongkam, Sakkarin
Paosen, Supakit
Bilhman, Siwaporn
Dumjun, Krittima
Wunnoo, Suttiwan
Choojit, Suntree
Siri, Ratchaneewan
Daengngam, Chalongrat
Voravuthikunchai, Supayang P.
Bejrananda, Tanan
Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title_full Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title_fullStr Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title_full_unstemmed Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title_short Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation
title_sort eucalyptus-mediated synthesized silver nanoparticles-coated urinary catheter inhibits microbial migration and biofilm formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699417/
https://www.ncbi.nlm.nih.gov/pubmed/36432345
http://dx.doi.org/10.3390/nano12224059
work_keys_str_mv AT lethongkamsakkarin eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT paosensupakit eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT bilhmansiwaporn eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT dumjunkrittima eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT wunnoosuttiwan eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT choojitsuntree eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT siriratchaneewan eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT daengngamchalongrat eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT voravuthikunchaisupayangp eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation
AT bejranandatanan eucalyptusmediatedsynthesizedsilvernanoparticlescoatedurinarycatheterinhibitsmicrobialmigrationandbiofilmformation