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Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiolo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699600/ https://www.ncbi.nlm.nih.gov/pubmed/36356031 http://dx.doi.org/10.3390/toxins14110780 |
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author | Morel, Jean-Luc Mokrzycki, Nathalie Lippens, Guy Drobecq, Hervé Sautière, Pierre Hugues, Michel |
author_facet | Morel, Jean-Luc Mokrzycki, Nathalie Lippens, Guy Drobecq, Hervé Sautière, Pierre Hugues, Michel |
author_sort | Morel, Jean-Luc |
collection | PubMed |
description | The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from Leuirus quinquestratus quinquestratus venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance. |
format | Online Article Text |
id | pubmed-9699600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96996002022-11-26 Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes Morel, Jean-Luc Mokrzycki, Nathalie Lippens, Guy Drobecq, Hervé Sautière, Pierre Hugues, Michel Toxins (Basel) Article The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from Leuirus quinquestratus quinquestratus venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance. MDPI 2022-11-10 /pmc/articles/PMC9699600/ /pubmed/36356031 http://dx.doi.org/10.3390/toxins14110780 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morel, Jean-Luc Mokrzycki, Nathalie Lippens, Guy Drobecq, Hervé Sautière, Pierre Hugues, Michel Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title | Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title_full | Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title_fullStr | Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title_full_unstemmed | Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title_short | Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes |
title_sort | characterization of a family of scorpion toxins modulating ca(2+)-activated cl(−) current in vascular myocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699600/ https://www.ncbi.nlm.nih.gov/pubmed/36356031 http://dx.doi.org/10.3390/toxins14110780 |
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