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Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes

The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiolo...

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Autores principales: Morel, Jean-Luc, Mokrzycki, Nathalie, Lippens, Guy, Drobecq, Hervé, Sautière, Pierre, Hugues, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699600/
https://www.ncbi.nlm.nih.gov/pubmed/36356031
http://dx.doi.org/10.3390/toxins14110780
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author Morel, Jean-Luc
Mokrzycki, Nathalie
Lippens, Guy
Drobecq, Hervé
Sautière, Pierre
Hugues, Michel
author_facet Morel, Jean-Luc
Mokrzycki, Nathalie
Lippens, Guy
Drobecq, Hervé
Sautière, Pierre
Hugues, Michel
author_sort Morel, Jean-Luc
collection PubMed
description The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from Leuirus quinquestratus quinquestratus venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance.
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spelling pubmed-96996002022-11-26 Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes Morel, Jean-Luc Mokrzycki, Nathalie Lippens, Guy Drobecq, Hervé Sautière, Pierre Hugues, Michel Toxins (Basel) Article The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from Leuirus quinquestratus quinquestratus venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance. MDPI 2022-11-10 /pmc/articles/PMC9699600/ /pubmed/36356031 http://dx.doi.org/10.3390/toxins14110780 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morel, Jean-Luc
Mokrzycki, Nathalie
Lippens, Guy
Drobecq, Hervé
Sautière, Pierre
Hugues, Michel
Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title_full Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title_fullStr Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title_full_unstemmed Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title_short Characterization of a Family of Scorpion Toxins Modulating Ca(2+)-Activated Cl(−) Current in Vascular Myocytes
title_sort characterization of a family of scorpion toxins modulating ca(2+)-activated cl(−) current in vascular myocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699600/
https://www.ncbi.nlm.nih.gov/pubmed/36356031
http://dx.doi.org/10.3390/toxins14110780
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