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Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells
We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulat...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699612/ https://www.ncbi.nlm.nih.gov/pubmed/36362982 http://dx.doi.org/10.3390/life12111827 |
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author | Wang, Wei Di Nisio, Elena Licursi, Valerio Cacci, Emanuele Lupo, Giuseppe Kokaia, Zaal Galanti, Sergio Degan, Paolo D’Angelo, Sara Castagnola, Patrizio Tavella, Sara Negri, Rodolfo |
author_facet | Wang, Wei Di Nisio, Elena Licursi, Valerio Cacci, Emanuele Lupo, Giuseppe Kokaia, Zaal Galanti, Sergio Degan, Paolo D’Angelo, Sara Castagnola, Patrizio Tavella, Sara Negri, Rodolfo |
author_sort | Wang, Wei |
collection | PubMed |
description | We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures. |
format | Online Article Text |
id | pubmed-9699612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96996122022-11-26 Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells Wang, Wei Di Nisio, Elena Licursi, Valerio Cacci, Emanuele Lupo, Giuseppe Kokaia, Zaal Galanti, Sergio Degan, Paolo D’Angelo, Sara Castagnola, Patrizio Tavella, Sara Negri, Rodolfo Life (Basel) Article We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures. MDPI 2022-11-09 /pmc/articles/PMC9699612/ /pubmed/36362982 http://dx.doi.org/10.3390/life12111827 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Wei Di Nisio, Elena Licursi, Valerio Cacci, Emanuele Lupo, Giuseppe Kokaia, Zaal Galanti, Sergio Degan, Paolo D’Angelo, Sara Castagnola, Patrizio Tavella, Sara Negri, Rodolfo Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title_full | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title_fullStr | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title_full_unstemmed | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title_short | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
title_sort | simulated microgravity modulates focal adhesion gene expression in human neural stem progenitor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699612/ https://www.ncbi.nlm.nih.gov/pubmed/36362982 http://dx.doi.org/10.3390/life12111827 |
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