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Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis
Local inflammation in the joint is considered to contribute to osteoarthritis (OA) progression. Here, we describe an immunomodulating nanoparticle for OA treatment. Intradermal injection of lipid nanoparticles (LNPs) loaded with type II collagen (Col II) and rapamycin (LNP-Col II-R) into OA mice eff...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699678/ https://www.ncbi.nlm.nih.gov/pubmed/36427299 http://dx.doi.org/10.1126/sciadv.abo5284 |
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author | Sohn, Hee Su Choi, Jeong Won Jhun, JooYeon Kwon, Sung Pil Jung, Mungyo Yong, Sangmin Na, Hyun Sik Kim, Jin-Hong Cho, Mi-La Kim, Byung-Soo |
author_facet | Sohn, Hee Su Choi, Jeong Won Jhun, JooYeon Kwon, Sung Pil Jung, Mungyo Yong, Sangmin Na, Hyun Sik Kim, Jin-Hong Cho, Mi-La Kim, Byung-Soo |
author_sort | Sohn, Hee Su |
collection | PubMed |
description | Local inflammation in the joint is considered to contribute to osteoarthritis (OA) progression. Here, we describe an immunomodulating nanoparticle for OA treatment. Intradermal injection of lipid nanoparticles (LNPs) loaded with type II collagen (Col II) and rapamycin (LNP-Col II-R) into OA mice effectively induced Col II–specific anti-inflammatory regulatory T cells, substantially increased anti-inflammatory cytokine expression, and reduced inflammatory immune cells and proinflammatory cytokine expression in the joints. Consequently, LNP-Col II-R injection inhibited chondrocyte apoptosis and cartilage matrix degradation and relieved pain, while injection of LNPs loaded with a control peptide and rapamycin did not induce these events. Adoptive transfer of CD4(+)CD25(+) T cells isolated from LNP-Col II-R–injected mice suggested that T(regs) induced by LNP-Col II-R injection were likely responsible for the therapeutic effects. Collectively, this study suggests nanoparticle-mediated immunomodulation in the joint as a simple and effective treatment for OA. |
format | Online Article Text |
id | pubmed-9699678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96996782022-12-05 Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis Sohn, Hee Su Choi, Jeong Won Jhun, JooYeon Kwon, Sung Pil Jung, Mungyo Yong, Sangmin Na, Hyun Sik Kim, Jin-Hong Cho, Mi-La Kim, Byung-Soo Sci Adv Biomedicine and Life Sciences Local inflammation in the joint is considered to contribute to osteoarthritis (OA) progression. Here, we describe an immunomodulating nanoparticle for OA treatment. Intradermal injection of lipid nanoparticles (LNPs) loaded with type II collagen (Col II) and rapamycin (LNP-Col II-R) into OA mice effectively induced Col II–specific anti-inflammatory regulatory T cells, substantially increased anti-inflammatory cytokine expression, and reduced inflammatory immune cells and proinflammatory cytokine expression in the joints. Consequently, LNP-Col II-R injection inhibited chondrocyte apoptosis and cartilage matrix degradation and relieved pain, while injection of LNPs loaded with a control peptide and rapamycin did not induce these events. Adoptive transfer of CD4(+)CD25(+) T cells isolated from LNP-Col II-R–injected mice suggested that T(regs) induced by LNP-Col II-R injection were likely responsible for the therapeutic effects. Collectively, this study suggests nanoparticle-mediated immunomodulation in the joint as a simple and effective treatment for OA. American Association for the Advancement of Science 2022-11-25 /pmc/articles/PMC9699678/ /pubmed/36427299 http://dx.doi.org/10.1126/sciadv.abo5284 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Sohn, Hee Su Choi, Jeong Won Jhun, JooYeon Kwon, Sung Pil Jung, Mungyo Yong, Sangmin Na, Hyun Sik Kim, Jin-Hong Cho, Mi-La Kim, Byung-Soo Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title | Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title_full | Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title_fullStr | Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title_full_unstemmed | Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title_short | Tolerogenic nanoparticles induce type II collagen–specific regulatory T cells and ameliorate osteoarthritis |
title_sort | tolerogenic nanoparticles induce type ii collagen–specific regulatory t cells and ameliorate osteoarthritis |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699678/ https://www.ncbi.nlm.nih.gov/pubmed/36427299 http://dx.doi.org/10.1126/sciadv.abo5284 |
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