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Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively

Endometriosis has been found to be closely related to autophagy. This study aimed to elucidate the possible mechanism of Bushen Wenyang Huayu Decoction (BWHD) in treating endometriosis (EMs) by targeting TLR4/NF-κB-mediated autophagy. Autologous grafting was used to generate the EMs model in rats. O...

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Autores principales: Li, Ying, Meng, Xin, Fu, Xinping, An, Mingli, Liu, Huimin, Ma, Yiming, Li, Qingxue, Hao, Guorong, Ma, Yucong, Zhang, Yu, Yang, Jian, Chen, Jingwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699773/
https://www.ncbi.nlm.nih.gov/pubmed/36437825
http://dx.doi.org/10.1155/2022/4263417
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author Li, Ying
Meng, Xin
Fu, Xinping
An, Mingli
Liu, Huimin
Ma, Yiming
Li, Qingxue
Hao, Guorong
Ma, Yucong
Zhang, Yu
Yang, Jian
Chen, Jingwei
author_facet Li, Ying
Meng, Xin
Fu, Xinping
An, Mingli
Liu, Huimin
Ma, Yiming
Li, Qingxue
Hao, Guorong
Ma, Yucong
Zhang, Yu
Yang, Jian
Chen, Jingwei
author_sort Li, Ying
collection PubMed
description Endometriosis has been found to be closely related to autophagy. This study aimed to elucidate the possible mechanism of Bushen Wenyang Huayu Decoction (BWHD) in treating endometriosis (EMs) by targeting TLR4/NF-κB-mediated autophagy. Autologous grafting was used to generate the EMs model in rats. Once the model was developed, BWHD high-dose and low-dose groups received intragastric administration of BWHD, and the gestrinone group served as a positive control. Immunofluorescence labeling and Western blotting were used for the protein expression of toll-like receptor 4 (TLR4), nuclear transcription factor-κB (NF-κB), Beclin-1, and selective autophagy connector protein P62 (P62). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze mRNA levels of TLR4, NF-κB, Beclin-1, and P62. We found that BWHD significantly reduced the size of ectopic lesions in rats with EMs, regulated reproductive hormone levels, and alleviated the cell autophagy level. It suggested that BWHD could be an effective treatment of EMs by targeting TLR4/NF-κB signaling pathway.
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spelling pubmed-96997732022-11-26 Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively Li, Ying Meng, Xin Fu, Xinping An, Mingli Liu, Huimin Ma, Yiming Li, Qingxue Hao, Guorong Ma, Yucong Zhang, Yu Yang, Jian Chen, Jingwei Evid Based Complement Alternat Med Research Article Endometriosis has been found to be closely related to autophagy. This study aimed to elucidate the possible mechanism of Bushen Wenyang Huayu Decoction (BWHD) in treating endometriosis (EMs) by targeting TLR4/NF-κB-mediated autophagy. Autologous grafting was used to generate the EMs model in rats. Once the model was developed, BWHD high-dose and low-dose groups received intragastric administration of BWHD, and the gestrinone group served as a positive control. Immunofluorescence labeling and Western blotting were used for the protein expression of toll-like receptor 4 (TLR4), nuclear transcription factor-κB (NF-κB), Beclin-1, and selective autophagy connector protein P62 (P62). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze mRNA levels of TLR4, NF-κB, Beclin-1, and P62. We found that BWHD significantly reduced the size of ectopic lesions in rats with EMs, regulated reproductive hormone levels, and alleviated the cell autophagy level. It suggested that BWHD could be an effective treatment of EMs by targeting TLR4/NF-κB signaling pathway. Hindawi 2022-11-18 /pmc/articles/PMC9699773/ /pubmed/36437825 http://dx.doi.org/10.1155/2022/4263417 Text en Copyright © 2022 Ying Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Ying
Meng, Xin
Fu, Xinping
An, Mingli
Liu, Huimin
Ma, Yiming
Li, Qingxue
Hao, Guorong
Ma, Yucong
Zhang, Yu
Yang, Jian
Chen, Jingwei
Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title_full Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title_fullStr Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title_full_unstemmed Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title_short Bushen Wenyang Huayu Decoction Targets TLR4/NF-κB Mediated Autophagy to Treat Endometriosis Effectively
title_sort bushen wenyang huayu decoction targets tlr4/nf-κb mediated autophagy to treat endometriosis effectively
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699773/
https://www.ncbi.nlm.nih.gov/pubmed/36437825
http://dx.doi.org/10.1155/2022/4263417
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