Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells

Understanding chromatin dynamics in red blood cells (RBCs) is critical for exploring the differentiation process and homeostasis maintenance during erythropoiesis. Here, we describe a protocol for isolation of zebrafish erythrocytes labelled with gata1:dsRed by fluorescence-activated cell sorting. W...

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Detalles Bibliográficos
Autores principales: Ding, Yanyan, Liu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700001/
https://www.ncbi.nlm.nih.gov/pubmed/36595911
http://dx.doi.org/10.1016/j.xpro.2022.101889
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author Ding, Yanyan
Liu, Feng
author_facet Ding, Yanyan
Liu, Feng
author_sort Ding, Yanyan
collection PubMed
description Understanding chromatin dynamics in red blood cells (RBCs) is critical for exploring the differentiation process and homeostasis maintenance during erythropoiesis. Here, we describe a protocol for isolation of zebrafish erythrocytes labelled with gata1:dsRed by fluorescence-activated cell sorting. We detail steps for ATAC-seq library construction from the isolated RBCs and describe how to analyze the quality of the library. The library can then be used to assay genome-wide chromatin accessibility in these RBCs. For complete details on the use and execution of this protocol, please refer to Ding et al. (2021).(1)
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spelling pubmed-97000012022-11-27 Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells Ding, Yanyan Liu, Feng STAR Protoc Protocol Understanding chromatin dynamics in red blood cells (RBCs) is critical for exploring the differentiation process and homeostasis maintenance during erythropoiesis. Here, we describe a protocol for isolation of zebrafish erythrocytes labelled with gata1:dsRed by fluorescence-activated cell sorting. We detail steps for ATAC-seq library construction from the isolated RBCs and describe how to analyze the quality of the library. The library can then be used to assay genome-wide chromatin accessibility in these RBCs. For complete details on the use and execution of this protocol, please refer to Ding et al. (2021).(1) Elsevier 2022-11-23 /pmc/articles/PMC9700001/ /pubmed/36595911 http://dx.doi.org/10.1016/j.xpro.2022.101889 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ding, Yanyan
Liu, Feng
Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title_full Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title_fullStr Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title_full_unstemmed Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title_short Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells
title_sort protocol for isolation and atac-seq library construction of zebrafish red blood cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700001/
https://www.ncbi.nlm.nih.gov/pubmed/36595911
http://dx.doi.org/10.1016/j.xpro.2022.101889
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