Protocol for the generation of human induced hepatic stem cells using Sendai virus vectors

Our recent study demonstrated the generation of induced tissue-specific stem/progenitor (iTS/iTP) cells by the transient overexpression of reprogramming factors combined with tissue-specific selection. Here, we present a protocol to reprogram human hepatocytes to generate human induced tissue-specif...

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Detalles Bibliográficos
Autores principales: Noguchi, Hirofumi, Nakashima, Yoshiki, Watanabe, Masami, Matsushita, Masayuki, Tsukahara, Masayoshi, Saitoh, Issei, Miyagi-Shiohira, Chika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700318/
https://www.ncbi.nlm.nih.gov/pubmed/36595898
http://dx.doi.org/10.1016/j.xpro.2022.101884
Descripción
Sumario:Our recent study demonstrated the generation of induced tissue-specific stem/progenitor (iTS/iTP) cells by the transient overexpression of reprogramming factors combined with tissue-specific selection. Here, we present a protocol to reprogram human hepatocytes to generate human induced tissue-specific liver stem (iTS-L) cells. Human hepatocytes are transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and c-MYC. iTS-L cells continuously express mRNA of hepatocyte-specific markers (HNF1β and HNF4α) and do not form teratomas. For complete details on the use and execution of this protocol, please refer to Nakashima et al. (2022).(1)

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