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Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR

Endogenous protein tagging, in contrast to exogenous overexpression of tagged proteins, allows to characterize specific protein functions under defined physiological or pathophysiological conditions without the influence of non-physiological protein levels. The development of generic and homology-in...

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Detalles Bibliográficos
Autores principales:	Seibert, Marcel, Kurrle, Nina, Stolp, Verena, Nürnberger, Heike, Tzschentke, Sandra, Börner, Leon, Wempe, Frank, Serve, Hubert, Schnütgen, Frank
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Method Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700324/ https://www.ncbi.nlm.nih.gov/pubmed/36444289 http://dx.doi.org/10.1016/j.mex.2022.101929

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