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Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus

Gestational diabetes mellitus (GDM) is a severe global issue that requires immediate attention. MicroRNA expression abnormalities are possibly disease-specific and may contribute to GDM pathological processes. To date, there is limited data on miRNA profiling in GDM, especially that involves a longi...

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Autores principales: Jamalpour, Sajad, Zain, Shamsul Mohd, Vazifehmand, Reza, Mohamed, Zahurin, Pung, Yuh Fen, Kamyab, Hesam, Omar, Siti Zawiah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700700/
https://www.ncbi.nlm.nih.gov/pubmed/36434110
http://dx.doi.org/10.1038/s41598-022-23816-3
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author Jamalpour, Sajad
Zain, Shamsul Mohd
Vazifehmand, Reza
Mohamed, Zahurin
Pung, Yuh Fen
Kamyab, Hesam
Omar, Siti Zawiah
author_facet Jamalpour, Sajad
Zain, Shamsul Mohd
Vazifehmand, Reza
Mohamed, Zahurin
Pung, Yuh Fen
Kamyab, Hesam
Omar, Siti Zawiah
author_sort Jamalpour, Sajad
collection PubMed
description Gestational diabetes mellitus (GDM) is a severe global issue that requires immediate attention. MicroRNA expression abnormalities are possibly disease-specific and may contribute to GDM pathological processes. To date, there is limited data on miRNA profiling in GDM, especially that involves a longitudinal study. Here, we performed miRNA expression profiling in the entire duration of pregnancy (during pregnancy until parturition and postpartum) using a miRNA- polymerase chain reaction array (miRNA-PCRArray) and in-silico analysis to identify unique miRNAs expression and their anticipated target genes in Malay maternal serum. MiRNA expression levels and their unique potential as biomarkers were explored in this work. In GDM patients, the expression levels of hsa-miR-193a, hsa-miR-21, hsa-miR-23a, and hsa-miR-361 were significantly increased, but miR-130a was significantly downregulated. The area under the curve (AUC) and receiver operating characteristic (ROC) curve study demonstrated that hsa-miR-193a (AUC = 0.89060 ± 04,470, P = 0.0001), hsa-miR-21 (AUC = 0.89500 ± 04,411, P = 0.0001), and miR-130a (AUC = 0.6939 ± 0.05845, P = 0.0025) had potential biomarker features in GDM. In-silico analysis also revealed that KLF (Kruppel-Like family of transcription factor), ZNF25 (Zinc finger protein 25), AFF4 (ALF transcription elongation factor 4), C1orf143 (long intergenic non-protein coding RNA 2869), SRSF2 (serine and arginine rich splicing factor 2), and ZNF655 (Zinc finger protein 655) were prominent genes targeted by the common nodes of miR23a, miR130, miR193a, miR21, and miR361.Our findings suggest that circulating microRNAs in the first trimester has the potential for GDM screening in the Malay population.
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spelling pubmed-97007002022-11-27 Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus Jamalpour, Sajad Zain, Shamsul Mohd Vazifehmand, Reza Mohamed, Zahurin Pung, Yuh Fen Kamyab, Hesam Omar, Siti Zawiah Sci Rep Article Gestational diabetes mellitus (GDM) is a severe global issue that requires immediate attention. MicroRNA expression abnormalities are possibly disease-specific and may contribute to GDM pathological processes. To date, there is limited data on miRNA profiling in GDM, especially that involves a longitudinal study. Here, we performed miRNA expression profiling in the entire duration of pregnancy (during pregnancy until parturition and postpartum) using a miRNA- polymerase chain reaction array (miRNA-PCRArray) and in-silico analysis to identify unique miRNAs expression and their anticipated target genes in Malay maternal serum. MiRNA expression levels and their unique potential as biomarkers were explored in this work. In GDM patients, the expression levels of hsa-miR-193a, hsa-miR-21, hsa-miR-23a, and hsa-miR-361 were significantly increased, but miR-130a was significantly downregulated. The area under the curve (AUC) and receiver operating characteristic (ROC) curve study demonstrated that hsa-miR-193a (AUC = 0.89060 ± 04,470, P = 0.0001), hsa-miR-21 (AUC = 0.89500 ± 04,411, P = 0.0001), and miR-130a (AUC = 0.6939 ± 0.05845, P = 0.0025) had potential biomarker features in GDM. In-silico analysis also revealed that KLF (Kruppel-Like family of transcription factor), ZNF25 (Zinc finger protein 25), AFF4 (ALF transcription elongation factor 4), C1orf143 (long intergenic non-protein coding RNA 2869), SRSF2 (serine and arginine rich splicing factor 2), and ZNF655 (Zinc finger protein 655) were prominent genes targeted by the common nodes of miR23a, miR130, miR193a, miR21, and miR361.Our findings suggest that circulating microRNAs in the first trimester has the potential for GDM screening in the Malay population. Nature Publishing Group UK 2022-11-24 /pmc/articles/PMC9700700/ /pubmed/36434110 http://dx.doi.org/10.1038/s41598-022-23816-3 Text en © The Author(s) 2022, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jamalpour, Sajad
Zain, Shamsul Mohd
Vazifehmand, Reza
Mohamed, Zahurin
Pung, Yuh Fen
Kamyab, Hesam
Omar, Siti Zawiah
Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title_full Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title_fullStr Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title_full_unstemmed Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title_short Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus
title_sort analysis of serum circulating micrornas level in malaysian patients with gestational diabetes mellitus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700700/
https://www.ncbi.nlm.nih.gov/pubmed/36434110
http://dx.doi.org/10.1038/s41598-022-23816-3
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