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A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo
Elucidation of spatial interactions between cancer and host cells is important for the development of new therapies against disseminated cancers. The aim of this study is to establish easy and useful method for elucidating spatial interactions. In this study, we developed a practical spatial analysi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700726/ https://www.ncbi.nlm.nih.gov/pubmed/36434071 http://dx.doi.org/10.1038/s41598-022-24827-w |
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author | Ota, Yukihide Sato, Shinya Yoshihara, Mitsuyo Nakamura, Yoshiyasu Miyagi, Etsuko Miyagi, Yohei |
author_facet | Ota, Yukihide Sato, Shinya Yoshihara, Mitsuyo Nakamura, Yoshiyasu Miyagi, Etsuko Miyagi, Yohei |
author_sort | Ota, Yukihide |
collection | PubMed |
description | Elucidation of spatial interactions between cancer and host cells is important for the development of new therapies against disseminated cancers. The aim of this study is to establish easy and useful method for elucidating spatial interactions. In this study, we developed a practical spatial analysis method using a gel-based embedding system and applied it to a murine model of cancer dissemination. After euthanization, every abdominal organ enclosed in the peritoneum was extracted en bloc. We injected agarose gel into the peritoneal cavities to preserve the spatial locations of the organs, including their metastatic niches, and then produced specimens when the gel had solidified. Preservation of the original spatial localization was confirmed by correlating magnetic resonance imaging results with the sectioned specimens. We examined the effects of spatial localization on cancer hypoxia using immunohistochemical hypoxia markers. Finally, we identified the mRNA expression of the specimens and demonstrated the applicability of spatial genetic analysis. In conclusion, we established a practical method for the in vivo investigation of spatial location-specific biological mechanisms in disseminated cancers. Our method can elucidate dissemination mechanisms, find therapeutic targets, and evaluate cancer therapeutic effects. |
format | Online Article Text |
id | pubmed-9700726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97007262022-11-27 A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo Ota, Yukihide Sato, Shinya Yoshihara, Mitsuyo Nakamura, Yoshiyasu Miyagi, Etsuko Miyagi, Yohei Sci Rep Article Elucidation of spatial interactions between cancer and host cells is important for the development of new therapies against disseminated cancers. The aim of this study is to establish easy and useful method for elucidating spatial interactions. In this study, we developed a practical spatial analysis method using a gel-based embedding system and applied it to a murine model of cancer dissemination. After euthanization, every abdominal organ enclosed in the peritoneum was extracted en bloc. We injected agarose gel into the peritoneal cavities to preserve the spatial locations of the organs, including their metastatic niches, and then produced specimens when the gel had solidified. Preservation of the original spatial localization was confirmed by correlating magnetic resonance imaging results with the sectioned specimens. We examined the effects of spatial localization on cancer hypoxia using immunohistochemical hypoxia markers. Finally, we identified the mRNA expression of the specimens and demonstrated the applicability of spatial genetic analysis. In conclusion, we established a practical method for the in vivo investigation of spatial location-specific biological mechanisms in disseminated cancers. Our method can elucidate dissemination mechanisms, find therapeutic targets, and evaluate cancer therapeutic effects. Nature Publishing Group UK 2022-11-24 /pmc/articles/PMC9700726/ /pubmed/36434071 http://dx.doi.org/10.1038/s41598-022-24827-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ota, Yukihide Sato, Shinya Yoshihara, Mitsuyo Nakamura, Yoshiyasu Miyagi, Etsuko Miyagi, Yohei A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title | A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title_full | A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title_fullStr | A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title_full_unstemmed | A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title_short | A practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
title_sort | practical spatial analysis method for elucidating the biological mechanisms of cancers with abdominal dissemination in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700726/ https://www.ncbi.nlm.nih.gov/pubmed/36434071 http://dx.doi.org/10.1038/s41598-022-24827-w |
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