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CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction

BACKGROUND: More and more evidence showed that circRNA/miRNA/mRNA axis played a vital role in the pathogenesis of some diseases. However, the role of circRNA/miRNA/mRNA axis in partial bladder outlet obstruction (pBOO) remains unknown. Our study aimed to explore the complex regulatory mechanism of c...

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Autores principales: Zhu, Baoyi, Gao, Jun, Zhang, Yuying, Liao, Baojian, Zhu, Sihua, Li, Chunling, Liao, Junhao, Liu, Jianjia, Jiang, Chonghe, Zeng, Jianwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700926/
https://www.ncbi.nlm.nih.gov/pubmed/36434693
http://dx.doi.org/10.1186/s12894-022-01132-2
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author Zhu, Baoyi
Gao, Jun
Zhang, Yuying
Liao, Baojian
Zhu, Sihua
Li, Chunling
Liao, Junhao
Liu, Jianjia
Jiang, Chonghe
Zeng, Jianwen
author_facet Zhu, Baoyi
Gao, Jun
Zhang, Yuying
Liao, Baojian
Zhu, Sihua
Li, Chunling
Liao, Junhao
Liu, Jianjia
Jiang, Chonghe
Zeng, Jianwen
author_sort Zhu, Baoyi
collection PubMed
description BACKGROUND: More and more evidence showed that circRNA/miRNA/mRNA axis played a vital role in the pathogenesis of some diseases. However, the role of circRNA/miRNA/mRNA axis in partial bladder outlet obstruction (pBOO) remains unknown. Our study aimed to explore the complex regulatory mechanism of circRNA/miRNA/mRNA axis in pBOO. METHODS: The pBOO rat model was established, and the bladder tissues were collected for mRNA sequencing. The differentially expressed mRNAs were analyzed by high-throughput sequencing, and the GO and KEGG analysis of the differentially expressed mRNAs were performed. Competing endogenous RNAs (ceRNAs) analysis identified the potential regulation function of circRNA/miRNA/mRNA axis in pBOO. qRT-PCR detected the expression of circRNA/miRNA/mRNA. miRanda software was performed to predict the relationship between circRNA and miRNA, miRNA and mRNA. RESULTS: Compared with the sham group, a total of 571 mRNAs were differentially expressed in the pBOO group, of which 286 were up-regulated and 285 were down-regulated. GO analysis showed that the mRNAs were mainly involved in cellular process, single-organism process, and cell, etc. KEGG analysis showed that the enriched signaling pathways were metabolic pathways, cell adhesion molecules (CAMs), and HTLV-I infection, etc. Based on the previous transcriptome data and differentially expressed circRNAs, we drew the ceRNA network regulation diagram. qRT-PCR results confirmed that chr3:113195876|113197193/rno-miR-30c-1-3p/Gata4, chr1:126188351|126195625/rno-miR-153-5p/Diaph3, and chr9:81258380|81275269/rno-miR-135b-5p/Pigr axis may have ceRNA function. miRanda confirmed there have the binding sites of circRNA/miRNA/mRNA axis. CONCLUSIONS: CircRNA/miRNA/mRNA axis was involved in the progression of pBOO. Our research on the circRNA/miRNA/mRNA axis revealed new pathogenesis and treatment strategies for pBOO.
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spelling pubmed-97009262022-11-27 CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction Zhu, Baoyi Gao, Jun Zhang, Yuying Liao, Baojian Zhu, Sihua Li, Chunling Liao, Junhao Liu, Jianjia Jiang, Chonghe Zeng, Jianwen BMC Urol Research BACKGROUND: More and more evidence showed that circRNA/miRNA/mRNA axis played a vital role in the pathogenesis of some diseases. However, the role of circRNA/miRNA/mRNA axis in partial bladder outlet obstruction (pBOO) remains unknown. Our study aimed to explore the complex regulatory mechanism of circRNA/miRNA/mRNA axis in pBOO. METHODS: The pBOO rat model was established, and the bladder tissues were collected for mRNA sequencing. The differentially expressed mRNAs were analyzed by high-throughput sequencing, and the GO and KEGG analysis of the differentially expressed mRNAs were performed. Competing endogenous RNAs (ceRNAs) analysis identified the potential regulation function of circRNA/miRNA/mRNA axis in pBOO. qRT-PCR detected the expression of circRNA/miRNA/mRNA. miRanda software was performed to predict the relationship between circRNA and miRNA, miRNA and mRNA. RESULTS: Compared with the sham group, a total of 571 mRNAs were differentially expressed in the pBOO group, of which 286 were up-regulated and 285 were down-regulated. GO analysis showed that the mRNAs were mainly involved in cellular process, single-organism process, and cell, etc. KEGG analysis showed that the enriched signaling pathways were metabolic pathways, cell adhesion molecules (CAMs), and HTLV-I infection, etc. Based on the previous transcriptome data and differentially expressed circRNAs, we drew the ceRNA network regulation diagram. qRT-PCR results confirmed that chr3:113195876|113197193/rno-miR-30c-1-3p/Gata4, chr1:126188351|126195625/rno-miR-153-5p/Diaph3, and chr9:81258380|81275269/rno-miR-135b-5p/Pigr axis may have ceRNA function. miRanda confirmed there have the binding sites of circRNA/miRNA/mRNA axis. CONCLUSIONS: CircRNA/miRNA/mRNA axis was involved in the progression of pBOO. Our research on the circRNA/miRNA/mRNA axis revealed new pathogenesis and treatment strategies for pBOO. BioMed Central 2022-11-25 /pmc/articles/PMC9700926/ /pubmed/36434693 http://dx.doi.org/10.1186/s12894-022-01132-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhu, Baoyi
Gao, Jun
Zhang, Yuying
Liao, Baojian
Zhu, Sihua
Li, Chunling
Liao, Junhao
Liu, Jianjia
Jiang, Chonghe
Zeng, Jianwen
CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title_full CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title_fullStr CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title_full_unstemmed CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title_short CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
title_sort circrna/mirna/mrna axis participates in the progression of partial bladder outlet obstruction
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700926/
https://www.ncbi.nlm.nih.gov/pubmed/36434693
http://dx.doi.org/10.1186/s12894-022-01132-2
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