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LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats

BACKGROUNDS: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease. Current studies suggest that long noncoding RNAs (lncRNAs) may be key regulators in pathogenesis. METHODS: Analyzed lncRNAs and mRNAs using microarrays to find key differentially expressed lncRNAs in RA. GO and...

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Autores principales: Wen, Yuqi, He, Cailin, Wang, Yang, Zeng, Siqin, Yang, Bo, Xiong, Xingui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700927/
https://www.ncbi.nlm.nih.gov/pubmed/36434596
http://dx.doi.org/10.1186/s12920-022-01398-3
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author Wen, Yuqi
He, Cailin
Wang, Yang
Zeng, Siqin
Yang, Bo
Xiong, Xingui
author_facet Wen, Yuqi
He, Cailin
Wang, Yang
Zeng, Siqin
Yang, Bo
Xiong, Xingui
author_sort Wen, Yuqi
collection PubMed
description BACKGROUNDS: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease. Current studies suggest that long noncoding RNAs (lncRNAs) may be key regulators in pathogenesis. METHODS: Analyzed lncRNAs and mRNAs using microarrays to find key differentially expressed lncRNAs in RA. GO and KEGG enrichment analysis together with coding non-coding co-expression (CNC) network was used for comprehensive analysis. Verify that their expression levels are consistent with the chip results by qRT-PCR. RESULTS: There are 268 differentially expressed lncRNAs (DELs) and 286 differentially expressed mRNAs (DEMs). We found 8 core lncRNAs through the CNC network. Eight highly significantly differentially expressed lncRNAs corrected with microarray profiles. The functions and associated pathways of significantly differentially expressed lncRNAs were predicted by GO and KEGG analysis. They may be involved in the pathogenesis of RA. CONCLUSION: The differential expression profiles of lncRNAs and mRNAs in the collagen-induced arthritis rat model preliminarily predicted functions through comprehensive analysis. However, its exact role and specific mechanism remain to be further studied. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-022-01398-3.
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spelling pubmed-97009272022-11-27 LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats Wen, Yuqi He, Cailin Wang, Yang Zeng, Siqin Yang, Bo Xiong, Xingui BMC Med Genomics Research BACKGROUNDS: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease. Current studies suggest that long noncoding RNAs (lncRNAs) may be key regulators in pathogenesis. METHODS: Analyzed lncRNAs and mRNAs using microarrays to find key differentially expressed lncRNAs in RA. GO and KEGG enrichment analysis together with coding non-coding co-expression (CNC) network was used for comprehensive analysis. Verify that their expression levels are consistent with the chip results by qRT-PCR. RESULTS: There are 268 differentially expressed lncRNAs (DELs) and 286 differentially expressed mRNAs (DEMs). We found 8 core lncRNAs through the CNC network. Eight highly significantly differentially expressed lncRNAs corrected with microarray profiles. The functions and associated pathways of significantly differentially expressed lncRNAs were predicted by GO and KEGG analysis. They may be involved in the pathogenesis of RA. CONCLUSION: The differential expression profiles of lncRNAs and mRNAs in the collagen-induced arthritis rat model preliminarily predicted functions through comprehensive analysis. However, its exact role and specific mechanism remain to be further studied. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-022-01398-3. BioMed Central 2022-11-26 /pmc/articles/PMC9700927/ /pubmed/36434596 http://dx.doi.org/10.1186/s12920-022-01398-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wen, Yuqi
He, Cailin
Wang, Yang
Zeng, Siqin
Yang, Bo
Xiong, Xingui
LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title_full LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title_fullStr LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title_full_unstemmed LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title_short LncRNA-mRNA co-expression analysis revealed 8 core lncRNAs in rheumatoid arthritis of collagen-induced arthritis rats
title_sort lncrna-mrna co-expression analysis revealed 8 core lncrnas in rheumatoid arthritis of collagen-induced arthritis rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700927/
https://www.ncbi.nlm.nih.gov/pubmed/36434596
http://dx.doi.org/10.1186/s12920-022-01398-3
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