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Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates

BACKGROUND: A mixture of phenol and guanidine isothiocyanate (“P/GI”, the principal components of TRIzol™ and similar products) is routinely used to isolate RNA, DNA, and proteins from a single specimen. In time-course experiments of cells grown in tissue culture, replicate wells are often harvested...

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Detalles Bibliográficos
Autores principales: Snedden, Madeline, Singh, Lavisha, Kyathanahalli, Chandrashekara, Hirsch, Emmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700959/
https://www.ncbi.nlm.nih.gov/pubmed/36434619
http://dx.doi.org/10.1186/s12896-022-00766-2

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