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Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla

BACKGROUND: This experimental study aimed to assess the effect of irradiation of red light-emitting diode (LED) and Diode low-level laser (LLL) on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs). MATERIALS AND METHODS: SCAPs were isolated from the human tooth roo...

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Autores principales: Rahmati, Afsaneh, Abbasi, Roshanak, Najafi, Rezvan, Rezaei-soufi, Loghman, Karkehabadi, Hamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701043/
https://www.ncbi.nlm.nih.gov/pubmed/36434589
http://dx.doi.org/10.1186/s12903-022-02574-8
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author Rahmati, Afsaneh
Abbasi, Roshanak
Najafi, Rezvan
Rezaei-soufi, Loghman
Karkehabadi, Hamed
author_facet Rahmati, Afsaneh
Abbasi, Roshanak
Najafi, Rezvan
Rezaei-soufi, Loghman
Karkehabadi, Hamed
author_sort Rahmati, Afsaneh
collection PubMed
description BACKGROUND: This experimental study aimed to assess the effect of irradiation of red light-emitting diode (LED) and Diode low-level laser (LLL) on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs). MATERIALS AND METHODS: SCAPs were isolated from the human tooth root. The experimental groups were subjected to 4 J/cm(2) diode low level laser and red LED irradiation in osteogenic medium. The control group did not receive any irradiation. Cell viability/proliferation of SCAPs was assessed by the methyl thiazolyl tetrazolium (MTT) assay on days 1 and 2 (n = 9). Osteogenic differentiation was evaluated by alizarin red staining (ARS) (n = 3), and expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR) (n = 12) on days 1 and 2. SPSS version 18 was used for data evaluation. The Kruskal-Wallis and Mann-Whitney tests were used to compare the groups at each time point. RESULTS: The MTT assay showed no significant difference in cell viability/proliferation of SCAPs in the low level laser, red LED, and control groups at 24 or 48 h (P < 0.001). The ARS assessment showed that low level laser and red LED irradiation enhanced osteogenic differentiation of SCAPs. low level laser and red LED irradiation both induced over-expression of osteogenic/dentinogenic genes including alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) in SCAPs. Up-regulation of genes was significantly greater in low level laser irradiation group than red LED group (P < 0.001). CONCLUSION: Diode low level laser irradiation with 4 J/cm(2) energy density and red LED irradiation enhanced osteogenic differentiation of SCAPs without adversely affecting cell viability.
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spelling pubmed-97010432022-11-27 Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla Rahmati, Afsaneh Abbasi, Roshanak Najafi, Rezvan Rezaei-soufi, Loghman Karkehabadi, Hamed BMC Oral Health Research BACKGROUND: This experimental study aimed to assess the effect of irradiation of red light-emitting diode (LED) and Diode low-level laser (LLL) on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs). MATERIALS AND METHODS: SCAPs were isolated from the human tooth root. The experimental groups were subjected to 4 J/cm(2) diode low level laser and red LED irradiation in osteogenic medium. The control group did not receive any irradiation. Cell viability/proliferation of SCAPs was assessed by the methyl thiazolyl tetrazolium (MTT) assay on days 1 and 2 (n = 9). Osteogenic differentiation was evaluated by alizarin red staining (ARS) (n = 3), and expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR) (n = 12) on days 1 and 2. SPSS version 18 was used for data evaluation. The Kruskal-Wallis and Mann-Whitney tests were used to compare the groups at each time point. RESULTS: The MTT assay showed no significant difference in cell viability/proliferation of SCAPs in the low level laser, red LED, and control groups at 24 or 48 h (P < 0.001). The ARS assessment showed that low level laser and red LED irradiation enhanced osteogenic differentiation of SCAPs. low level laser and red LED irradiation both induced over-expression of osteogenic/dentinogenic genes including alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) in SCAPs. Up-regulation of genes was significantly greater in low level laser irradiation group than red LED group (P < 0.001). CONCLUSION: Diode low level laser irradiation with 4 J/cm(2) energy density and red LED irradiation enhanced osteogenic differentiation of SCAPs without adversely affecting cell viability. BioMed Central 2022-11-25 /pmc/articles/PMC9701043/ /pubmed/36434589 http://dx.doi.org/10.1186/s12903-022-02574-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Rahmati, Afsaneh
Abbasi, Roshanak
Najafi, Rezvan
Rezaei-soufi, Loghman
Karkehabadi, Hamed
Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title_full Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title_fullStr Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title_full_unstemmed Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title_short Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
title_sort effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701043/
https://www.ncbi.nlm.nih.gov/pubmed/36434589
http://dx.doi.org/10.1186/s12903-022-02574-8
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