Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN(+)) provide enhanced scalability for biomanufacturing. However, engineering enzymes to accept NCBs is difficult. Here, we establish a growth selection platform to evolve enzymes to utilize NMN(+)-based reducing power....

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Detalles Bibliográficos
Autores principales: King, Edward, Maxel, Sarah, Zhang, Yulai, Kenney, Karissa C., Cui, Youtian, Luu, Emma, Siegel, Justin B., Weiss, Gregory A., Luo, Ray, Li, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701214/
https://www.ncbi.nlm.nih.gov/pubmed/36435948
http://dx.doi.org/10.1038/s41467-022-35021-x

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