CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin

Gap Junction intercellular communication (GJIC) is often dysregulated in cancers, and this dysregulation has been shown to have pro-tumorigenic effects. Connexins (Cxs) are transmembrane proteins that make up gap junctions. Previous studies have indicated that RNA interference (RNAi)-based suppressi...

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Detalles Bibliográficos
Autores principales: Martin, Stephen, Mu, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701319/
https://www.ncbi.nlm.nih.gov/pubmed/36447529
http://dx.doi.org/10.17912/micropub.biology.000681
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author Martin, Stephen
Mu, David
author_facet Martin, Stephen
Mu, David
author_sort Martin, Stephen
collection PubMed
description Gap Junction intercellular communication (GJIC) is often dysregulated in cancers, and this dysregulation has been shown to have pro-tumorigenic effects. Connexins (Cxs) are transmembrane proteins that make up gap junctions. Previous studies have indicated that RNA interference (RNAi)-based suppression of Cx43 increases cellular resistance to the chemotherapeutic agent cisplatin. Interestingly, we found that the loss of Cx43 expression induced by the CRISPR-Cas9 technology sensitizes cells to cisplatin in a KRAS mutant-dependent manner.
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spelling pubmed-97013192022-11-28 CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin Martin, Stephen Mu, David MicroPubl Biol New Finding Gap Junction intercellular communication (GJIC) is often dysregulated in cancers, and this dysregulation has been shown to have pro-tumorigenic effects. Connexins (Cxs) are transmembrane proteins that make up gap junctions. Previous studies have indicated that RNA interference (RNAi)-based suppression of Cx43 increases cellular resistance to the chemotherapeutic agent cisplatin. Interestingly, we found that the loss of Cx43 expression induced by the CRISPR-Cas9 technology sensitizes cells to cisplatin in a KRAS mutant-dependent manner. Caltech Library 2022-11-13 /pmc/articles/PMC9701319/ /pubmed/36447529 http://dx.doi.org/10.17912/micropub.biology.000681 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Martin, Stephen
Mu, David
CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title_full CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title_fullStr CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title_full_unstemmed CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title_short CRISPR-Induced Loss of Connexin 43 Expression Sensitizes KRAS Mutant Cells to Cisplatin
title_sort crispr-induced loss of connexin 43 expression sensitizes kras mutant cells to cisplatin
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701319/
https://www.ncbi.nlm.nih.gov/pubmed/36447529
http://dx.doi.org/10.17912/micropub.biology.000681
work_keys_str_mv AT martinstephen crisprinducedlossofconnexin43expressionsensitizeskrasmutantcellstocisplatin
AT mudavid crisprinducedlossofconnexin43expressionsensitizeskrasmutantcellstocisplatin

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