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Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
BACKGROUND: Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion including th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701414/ https://www.ncbi.nlm.nih.gov/pubmed/36437462 http://dx.doi.org/10.1186/s12936-022-04382-5 |
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author | Win, Khine Nwe Manopwisedjaroen, Khajohnpong Phumchuea, Kanit Suansomjit, Chayanut Chotivanich, Kesinee Lawpoolsri, Saranath Cui, Liwang Sattabongkot, Jetsumon Nguitragool, Wang |
author_facet | Win, Khine Nwe Manopwisedjaroen, Khajohnpong Phumchuea, Kanit Suansomjit, Chayanut Chotivanich, Kesinee Lawpoolsri, Saranath Cui, Liwang Sattabongkot, Jetsumon Nguitragool, Wang |
author_sort | Win, Khine Nwe |
collection | PubMed |
description | BACKGROUND: Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion including the eastern part of Thailand near Cambodia. This study aimed to assess whether the resistance genotypes have arisen the western part of country. METHODS: Fifty-seven P. falciparum-infected blood samples were collected in Tak province of northwestern Thailand between 2013 and 2019. Resistance to DHA was examined through the single nucleotide polymorphisms (SNPs) of kelch13. PPQ resistance was examined through the copy number plasmepsin-2 and the SNPs of Pfcrt. RESULTS: Among the samples whose kelch13 were successfully sequenced, approximately half (31/55; 56%) had mutation associated with artemisinin resistance, including G533S (23/55; 42%), C580Y (6/55; 11%), and G538V (2/55; 4%). During the study period, G533S mutation appeared and increased from 20% (4/20) in 2014 to 100% (9/9) in 2019. No plasmepsin-2 gene amplification was observed, but one sample (1/54) had the Pfcrt F145I mutation previously implicated in PPQ resistance. CONCLUSIONS: Kelch13 mutation was common in Tak Province in 2013–2019. A new mutation G533S emerged in 2014 and rose to dominance in 2019. PPQ resistance marker Pfcrt F145I was also detected in 2019. Continued surveillance of treatment efficacy and drug resistance markers is warranted. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-022-04382-5. |
format | Online Article Text |
id | pubmed-9701414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97014142022-11-28 Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand Win, Khine Nwe Manopwisedjaroen, Khajohnpong Phumchuea, Kanit Suansomjit, Chayanut Chotivanich, Kesinee Lawpoolsri, Saranath Cui, Liwang Sattabongkot, Jetsumon Nguitragool, Wang Malar J Research BACKGROUND: Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion including the eastern part of Thailand near Cambodia. This study aimed to assess whether the resistance genotypes have arisen the western part of country. METHODS: Fifty-seven P. falciparum-infected blood samples were collected in Tak province of northwestern Thailand between 2013 and 2019. Resistance to DHA was examined through the single nucleotide polymorphisms (SNPs) of kelch13. PPQ resistance was examined through the copy number plasmepsin-2 and the SNPs of Pfcrt. RESULTS: Among the samples whose kelch13 were successfully sequenced, approximately half (31/55; 56%) had mutation associated with artemisinin resistance, including G533S (23/55; 42%), C580Y (6/55; 11%), and G538V (2/55; 4%). During the study period, G533S mutation appeared and increased from 20% (4/20) in 2014 to 100% (9/9) in 2019. No plasmepsin-2 gene amplification was observed, but one sample (1/54) had the Pfcrt F145I mutation previously implicated in PPQ resistance. CONCLUSIONS: Kelch13 mutation was common in Tak Province in 2013–2019. A new mutation G533S emerged in 2014 and rose to dominance in 2019. PPQ resistance marker Pfcrt F145I was also detected in 2019. Continued surveillance of treatment efficacy and drug resistance markers is warranted. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-022-04382-5. BioMed Central 2022-11-27 /pmc/articles/PMC9701414/ /pubmed/36437462 http://dx.doi.org/10.1186/s12936-022-04382-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Win, Khine Nwe Manopwisedjaroen, Khajohnpong Phumchuea, Kanit Suansomjit, Chayanut Chotivanich, Kesinee Lawpoolsri, Saranath Cui, Liwang Sattabongkot, Jetsumon Nguitragool, Wang Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title | Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title_full | Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title_fullStr | Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title_full_unstemmed | Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title_short | Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand |
title_sort | molecular markers of dihydroartemisinin-piperaquine resistance in northwestern thailand |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701414/ https://www.ncbi.nlm.nih.gov/pubmed/36437462 http://dx.doi.org/10.1186/s12936-022-04382-5 |
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