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Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts
This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in toma...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701672/ https://www.ncbi.nlm.nih.gov/pubmed/36437281 http://dx.doi.org/10.1038/s41598-022-24880-5 |
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author | McLaughlin, Autumn A. Hanley-Bowdoin, Linda Kennedy, George G. Jacobson, Alana L. |
author_facet | McLaughlin, Autumn A. Hanley-Bowdoin, Linda Kennedy, George G. Jacobson, Alana L. |
author_sort | McLaughlin, Autumn A. |
collection | PubMed |
description | This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in tomato. The aim of this research was to determine if the manner in which viruses are co-acquired and co-transmitted changes the probability of acquisition, transmission and new host infections. Whiteflies acquired virus by feeding on singly infected plants, co-infected plants, or by sequential feeding on singly infected plants. Viral titers were also quantified by qPCR in vector cohorts, in artificial diet, and plants after exposure to viruliferous vectors. Differences in transmission, infection status of plants, and titers of TYLCV and ToMoV were observed among treatments. All vector cohorts acquired both viruses, but co-acquisition/co-inoculation generally reduced transmission of both viruses as single and mixed infections. Co-inoculation of viruses by the vector also altered virus accumulation in plants regardless of whether one or both viruses were propagated in new hosts. These findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections. |
format | Online Article Text |
id | pubmed-9701672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97016722022-11-29 Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts McLaughlin, Autumn A. Hanley-Bowdoin, Linda Kennedy, George G. Jacobson, Alana L. Sci Rep Article This study investigated the role of vector acquisition and transmission on the propagation of single and co-infections of tomato yellow leaf curl virus (TYLCV,) and tomato mottle virus (ToMoV) (Family: Geminiviridae, Genus: Begomovirus) by the whitefly vector Bemisia tabaci MEAM1 (Gennadius) in tomato. The aim of this research was to determine if the manner in which viruses are co-acquired and co-transmitted changes the probability of acquisition, transmission and new host infections. Whiteflies acquired virus by feeding on singly infected plants, co-infected plants, or by sequential feeding on singly infected plants. Viral titers were also quantified by qPCR in vector cohorts, in artificial diet, and plants after exposure to viruliferous vectors. Differences in transmission, infection status of plants, and titers of TYLCV and ToMoV were observed among treatments. All vector cohorts acquired both viruses, but co-acquisition/co-inoculation generally reduced transmission of both viruses as single and mixed infections. Co-inoculation of viruses by the vector also altered virus accumulation in plants regardless of whether one or both viruses were propagated in new hosts. These findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections. Nature Publishing Group UK 2022-11-27 /pmc/articles/PMC9701672/ /pubmed/36437281 http://dx.doi.org/10.1038/s41598-022-24880-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article McLaughlin, Autumn A. Hanley-Bowdoin, Linda Kennedy, George G. Jacobson, Alana L. Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title_full | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title_fullStr | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title_full_unstemmed | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title_short | Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
title_sort | vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701672/ https://www.ncbi.nlm.nih.gov/pubmed/36437281 http://dx.doi.org/10.1038/s41598-022-24880-5 |
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