Cargando…

Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen

In this study we aimed to determine whether treatment with maternal Bifidobacterium bifidum TMC3115 could affect the composition of the gut microbiota and the development of the immune system and intestinal tract of offspring, and protect the offspring from IgE-mediated allergic disease. Pregnant BA...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Ruyue, Zhang, Yujie, Yang, Yang, Ren, Lei, Li, Jinxing, Wang, Yimei, Shen, Xi, He, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701730/
https://www.ncbi.nlm.nih.gov/pubmed/36452299
http://dx.doi.org/10.3389/fcimb.2022.1045109
_version_ 1784839600517152768
author Cheng, Ruyue
Zhang, Yujie
Yang, Yang
Ren, Lei
Li, Jinxing
Wang, Yimei
Shen, Xi
He, Fang
author_facet Cheng, Ruyue
Zhang, Yujie
Yang, Yang
Ren, Lei
Li, Jinxing
Wang, Yimei
Shen, Xi
He, Fang
author_sort Cheng, Ruyue
collection PubMed
description In this study we aimed to determine whether treatment with maternal Bifidobacterium bifidum TMC3115 could affect the composition of the gut microbiota and the development of the immune system and intestinal tract of offspring, and protect the offspring from IgE-mediated allergic disease. Pregnant BALB/c mice were gavaged with TMC3115 until delivery. Offspring were sensitized with ovalbumin from postnatal days 21 to 49. After maternal treatment with TMC3115, the microbiota of the offspring’s feces, intestinal contents, and stomach contents (a proxy for breast milk) at the newborn and weaning stages exhibited the most change, and levels of immunoglobulin in the sera and stomach contents and of splenic cytokines, as well as the mRNA levels of colonic intestinal development indicators were all significantly altered in offspring at different stages. After sensitization with ovalbumin, there were no significant changes in the levels of serum IgE or ovalbumin-specific IgE/IgG1 in the TMC3115 group; however, IgM, the expression of intestinal development indicators, and the production of fecal short chain fatty acid (SCFA) were significantly increased, as were the relative abundances of Lactobacillus and the Lachnospiraceae NK4A136 group. Our results suggested that maternal treatment with TMC3115 could have a profound modulatory effect on the composition of the gut microbiota and the development of the immune system and intestinal tissue in offspring at different stages of development, and may induce immune tolerance to allergens in ovalbumin-stimulated offspring by modulating the gut microbiota and SCFA production.
format Online
Article
Text
id pubmed-9701730
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97017302022-11-29 Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen Cheng, Ruyue Zhang, Yujie Yang, Yang Ren, Lei Li, Jinxing Wang, Yimei Shen, Xi He, Fang Front Cell Infect Microbiol Cellular and Infection Microbiology In this study we aimed to determine whether treatment with maternal Bifidobacterium bifidum TMC3115 could affect the composition of the gut microbiota and the development of the immune system and intestinal tract of offspring, and protect the offspring from IgE-mediated allergic disease. Pregnant BALB/c mice were gavaged with TMC3115 until delivery. Offspring were sensitized with ovalbumin from postnatal days 21 to 49. After maternal treatment with TMC3115, the microbiota of the offspring’s feces, intestinal contents, and stomach contents (a proxy for breast milk) at the newborn and weaning stages exhibited the most change, and levels of immunoglobulin in the sera and stomach contents and of splenic cytokines, as well as the mRNA levels of colonic intestinal development indicators were all significantly altered in offspring at different stages. After sensitization with ovalbumin, there were no significant changes in the levels of serum IgE or ovalbumin-specific IgE/IgG1 in the TMC3115 group; however, IgM, the expression of intestinal development indicators, and the production of fecal short chain fatty acid (SCFA) were significantly increased, as were the relative abundances of Lactobacillus and the Lachnospiraceae NK4A136 group. Our results suggested that maternal treatment with TMC3115 could have a profound modulatory effect on the composition of the gut microbiota and the development of the immune system and intestinal tissue in offspring at different stages of development, and may induce immune tolerance to allergens in ovalbumin-stimulated offspring by modulating the gut microbiota and SCFA production. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9701730/ /pubmed/36452299 http://dx.doi.org/10.3389/fcimb.2022.1045109 Text en Copyright © 2022 Cheng, Zhang, Yang, Ren, Li, Wang, Shen and He https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Cheng, Ruyue
Zhang, Yujie
Yang, Yang
Ren, Lei
Li, Jinxing
Wang, Yimei
Shen, Xi
He, Fang
Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title_full Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title_fullStr Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title_full_unstemmed Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title_short Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
title_sort maternal gestational bifidobacterium bifidum tmc3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701730/
https://www.ncbi.nlm.nih.gov/pubmed/36452299
http://dx.doi.org/10.3389/fcimb.2022.1045109
work_keys_str_mv AT chengruyue maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT zhangyujie maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT yangyang maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT renlei maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT lijinxing maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT wangyimei maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT shenxi maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen
AT hefang maternalgestationalbifidobacteriumbifidumtmc3115treatmentshapesconstructionofoffspringgutmicrobiotaanddevelopmentofimmunesystemandinducesimmunetolerancetofoodallergen