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Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis
Eosinophilic esophagitis (EoE) is a chronic allergy-mediated condition with an increasing incidence in both children and adults. Despite EoE's strong impact on human health and welfare, there is a large unmet need for treatments with only one recently FDA-approved medication for EoE. The goal o...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701751/ https://www.ncbi.nlm.nih.gov/pubmed/36452260 http://dx.doi.org/10.3389/falgy.2022.1029184 |
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author | Cortes, Lizette M. Brodsky, David Chen, Celine Pridgen, Tiffany Odle, Jack Snider, Douglas B. Cruse, Glenn Putikova, Arina Masuda, Mia Y. Doyle, Alfred D. Wright, Benjamin L. Dawson, Harry D. Blikslager, Anthony Dellon, Evan S. Laster, Scott M. Käser, Tobias |
author_facet | Cortes, Lizette M. Brodsky, David Chen, Celine Pridgen, Tiffany Odle, Jack Snider, Douglas B. Cruse, Glenn Putikova, Arina Masuda, Mia Y. Doyle, Alfred D. Wright, Benjamin L. Dawson, Harry D. Blikslager, Anthony Dellon, Evan S. Laster, Scott M. Käser, Tobias |
author_sort | Cortes, Lizette M. |
collection | PubMed |
description | Eosinophilic esophagitis (EoE) is a chronic allergy-mediated condition with an increasing incidence in both children and adults. Despite EoE's strong impact on human health and welfare, there is a large unmet need for treatments with only one recently FDA-approved medication for EoE. The goal of this study was to establish swine as a relevant large animal model for translational biomedical research in EoE with the potential to facilitate development of therapeutics. We recently showed that after intraperitoneal sensitization and oral challenge with the food allergen hen egg white protein (HEWP), swine develop esophageal eosinophilia—a hallmark of human EoE. Herein, we used a similar sensitization and challenge treatment and evaluated immunological and pathological markers associated with human EoE. Our data demonstrate that the incorporated sensitization and challenge treatment induces (i) a systemic T-helper 2 and IgE response, (ii) a local expression of eotaxin-1 and other allergy-related immune markers, (iii) esophageal eosinophilia (>15 eosinophils/0.24 mm(2)), and (iv) esophageal endoscopic findings including linear furrows and white exudates. Thereby, we demonstrate that our sensitization and oral challenge protocol not only induces the underlying immune markers but also the micro- and macro-pathological hallmarks of human EoE. This swine model for EoE represents a novel relevant large animal model that can drive translational biomedical research to develop urgently needed treatment strategies for EoE. |
format | Online Article Text |
id | pubmed-9701751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97017512022-11-29 Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis Cortes, Lizette M. Brodsky, David Chen, Celine Pridgen, Tiffany Odle, Jack Snider, Douglas B. Cruse, Glenn Putikova, Arina Masuda, Mia Y. Doyle, Alfred D. Wright, Benjamin L. Dawson, Harry D. Blikslager, Anthony Dellon, Evan S. Laster, Scott M. Käser, Tobias Front Allergy Allergy Eosinophilic esophagitis (EoE) is a chronic allergy-mediated condition with an increasing incidence in both children and adults. Despite EoE's strong impact on human health and welfare, there is a large unmet need for treatments with only one recently FDA-approved medication for EoE. The goal of this study was to establish swine as a relevant large animal model for translational biomedical research in EoE with the potential to facilitate development of therapeutics. We recently showed that after intraperitoneal sensitization and oral challenge with the food allergen hen egg white protein (HEWP), swine develop esophageal eosinophilia—a hallmark of human EoE. Herein, we used a similar sensitization and challenge treatment and evaluated immunological and pathological markers associated with human EoE. Our data demonstrate that the incorporated sensitization and challenge treatment induces (i) a systemic T-helper 2 and IgE response, (ii) a local expression of eotaxin-1 and other allergy-related immune markers, (iii) esophageal eosinophilia (>15 eosinophils/0.24 mm(2)), and (iv) esophageal endoscopic findings including linear furrows and white exudates. Thereby, we demonstrate that our sensitization and oral challenge protocol not only induces the underlying immune markers but also the micro- and macro-pathological hallmarks of human EoE. This swine model for EoE represents a novel relevant large animal model that can drive translational biomedical research to develop urgently needed treatment strategies for EoE. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9701751/ /pubmed/36452260 http://dx.doi.org/10.3389/falgy.2022.1029184 Text en © 2022 Cortes, Brodsky, Chen, Pridgen, Odle, Snider, Cruse, Putikova, Masuda, Doyle, Wright, Dawson, Blikslager, Dellon, Laster and Käser. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Allergy Cortes, Lizette M. Brodsky, David Chen, Celine Pridgen, Tiffany Odle, Jack Snider, Douglas B. Cruse, Glenn Putikova, Arina Masuda, Mia Y. Doyle, Alfred D. Wright, Benjamin L. Dawson, Harry D. Blikslager, Anthony Dellon, Evan S. Laster, Scott M. Käser, Tobias Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title | Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title_full | Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title_fullStr | Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title_full_unstemmed | Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title_short | Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
title_sort | immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis |
topic | Allergy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701751/ https://www.ncbi.nlm.nih.gov/pubmed/36452260 http://dx.doi.org/10.3389/falgy.2022.1029184 |
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