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Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients

The present study applied distinct models of descriptive analysis to explore the integrative networks and the kinetic timeline of serum soluble mediators to select a set of systemic biomarkers applicable for the clinical management of COVID-19 patients. For this purpose, a total of 246 participants...

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Autores principales: Jardim-Santos, Gabriela Profírio, Schulte, Heidi Luise, Kurizky, Patricia Shu, Gomes, Ciro Martins, Nóbrega, Otávio Tolêdo, de Gois, Eliana Teles, de Carvalho, Maíra Rocha Machado, Martins, Francielle Pulccinelli, Nicola, André Moraes, de Albuquerque, Cleandro Pires, Espindola, Laila Salmen, Naves, Luciana Ansaneli, Soares, Alexandre Anderson de Sousa Munhoz, Albuquerque, Patrícia, Fontes, Wagner, do Amaral, Laurence Rodrigues, Gomes, Matheus de Souza, Bertarini, Pedro Luiz Lima, Brito-de-Sousa, Joaquim Pedro, Campi-Azevedo, Ana Carolina, Peruhype-Magalhães, Vanessa, Teixeira-Carvalho, Andrea, Valim, Valéria, Martins-Filho, Olindo Assis, da Mota, Licia Maria Henrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701840/
https://www.ncbi.nlm.nih.gov/pubmed/36451835
http://dx.doi.org/10.3389/fimmu.2022.1004023
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author Jardim-Santos, Gabriela Profírio
Schulte, Heidi Luise
Kurizky, Patricia Shu
Gomes, Ciro Martins
Nóbrega, Otávio Tolêdo
de Gois, Eliana Teles
de Carvalho, Maíra Rocha Machado
Martins, Francielle Pulccinelli
Nicola, André Moraes
de Albuquerque, Cleandro Pires
Espindola, Laila Salmen
Naves, Luciana Ansaneli
Soares, Alexandre Anderson de Sousa Munhoz
Albuquerque, Patrícia
Fontes, Wagner
do Amaral, Laurence Rodrigues
Gomes, Matheus de Souza
Bertarini, Pedro Luiz Lima
Brito-de-Sousa, Joaquim Pedro
Campi-Azevedo, Ana Carolina
Peruhype-Magalhães, Vanessa
Teixeira-Carvalho, Andrea
Valim, Valéria
Martins-Filho, Olindo Assis
da Mota, Licia Maria Henrique
author_facet Jardim-Santos, Gabriela Profírio
Schulte, Heidi Luise
Kurizky, Patricia Shu
Gomes, Ciro Martins
Nóbrega, Otávio Tolêdo
de Gois, Eliana Teles
de Carvalho, Maíra Rocha Machado
Martins, Francielle Pulccinelli
Nicola, André Moraes
de Albuquerque, Cleandro Pires
Espindola, Laila Salmen
Naves, Luciana Ansaneli
Soares, Alexandre Anderson de Sousa Munhoz
Albuquerque, Patrícia
Fontes, Wagner
do Amaral, Laurence Rodrigues
Gomes, Matheus de Souza
Bertarini, Pedro Luiz Lima
Brito-de-Sousa, Joaquim Pedro
Campi-Azevedo, Ana Carolina
Peruhype-Magalhães, Vanessa
Teixeira-Carvalho, Andrea
Valim, Valéria
Martins-Filho, Olindo Assis
da Mota, Licia Maria Henrique
author_sort Jardim-Santos, Gabriela Profírio
collection PubMed
description The present study applied distinct models of descriptive analysis to explore the integrative networks and the kinetic timeline of serum soluble mediators to select a set of systemic biomarkers applicable for the clinical management of COVID-19 patients. For this purpose, a total of 246 participants (82 COVID-19 and 164 healthy controls – HC) were enrolled in a prospective observational study. Serum soluble mediators were quantified by high-throughput microbeads array on hospital admission (D0) and at consecutive timepoints (D1-6 and D7-20). The results reinforce that the COVID-19 group exhibited a massive storm of serum soluble mediators. While increased levels of CCL3 and G-CSF were associated with the favorable prognosis of non-mechanical ventilation (nMV) or discharge, high levels of CXCL10 and IL-6 were observed in patients progressing to mechanical ventilation (MV) or death. At the time of admission, COVID-19 patients presented a complex and robust serum soluble mediator network, with a higher number of strong correlations involving IFN-γ, IL-1Ra and IL-9 observed in patients progressing to MV or death. Multivariate regression analysis demonstrates the ability of serum soluble mediators to cluster COVID-19 from HC. Ascendant fold change signatures and the kinetic timeline analysis further confirmed that the pairs “CCL3 and G-CSF” and “CXCL10 and IL-6” were associated with favorable or poor prognosis, respectively. A selected set of systemic mediators (IL-6, IFN-γ, IL-1Ra, IL-13, PDGF and IL-7) were identified as putative laboratory markers, applicable as complementary records for the clinical management of patients with severe COVID-19.
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spelling pubmed-97018402022-11-29 Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients Jardim-Santos, Gabriela Profírio Schulte, Heidi Luise Kurizky, Patricia Shu Gomes, Ciro Martins Nóbrega, Otávio Tolêdo de Gois, Eliana Teles de Carvalho, Maíra Rocha Machado Martins, Francielle Pulccinelli Nicola, André Moraes de Albuquerque, Cleandro Pires Espindola, Laila Salmen Naves, Luciana Ansaneli Soares, Alexandre Anderson de Sousa Munhoz Albuquerque, Patrícia Fontes, Wagner do Amaral, Laurence Rodrigues Gomes, Matheus de Souza Bertarini, Pedro Luiz Lima Brito-de-Sousa, Joaquim Pedro Campi-Azevedo, Ana Carolina Peruhype-Magalhães, Vanessa Teixeira-Carvalho, Andrea Valim, Valéria Martins-Filho, Olindo Assis da Mota, Licia Maria Henrique Front Immunol Immunology The present study applied distinct models of descriptive analysis to explore the integrative networks and the kinetic timeline of serum soluble mediators to select a set of systemic biomarkers applicable for the clinical management of COVID-19 patients. For this purpose, a total of 246 participants (82 COVID-19 and 164 healthy controls – HC) were enrolled in a prospective observational study. Serum soluble mediators were quantified by high-throughput microbeads array on hospital admission (D0) and at consecutive timepoints (D1-6 and D7-20). The results reinforce that the COVID-19 group exhibited a massive storm of serum soluble mediators. While increased levels of CCL3 and G-CSF were associated with the favorable prognosis of non-mechanical ventilation (nMV) or discharge, high levels of CXCL10 and IL-6 were observed in patients progressing to mechanical ventilation (MV) or death. At the time of admission, COVID-19 patients presented a complex and robust serum soluble mediator network, with a higher number of strong correlations involving IFN-γ, IL-1Ra and IL-9 observed in patients progressing to MV or death. Multivariate regression analysis demonstrates the ability of serum soluble mediators to cluster COVID-19 from HC. Ascendant fold change signatures and the kinetic timeline analysis further confirmed that the pairs “CCL3 and G-CSF” and “CXCL10 and IL-6” were associated with favorable or poor prognosis, respectively. A selected set of systemic mediators (IL-6, IFN-γ, IL-1Ra, IL-13, PDGF and IL-7) were identified as putative laboratory markers, applicable as complementary records for the clinical management of patients with severe COVID-19. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9701840/ /pubmed/36451835 http://dx.doi.org/10.3389/fimmu.2022.1004023 Text en Copyright © 2022 Jardim-Santos, Schulte, Kurizky, Gomes, Nóbrega, de Gois, de Carvalho, Martins, Nicola, de Albuquerque, Espindola, Naves, Soares, Albuquerque, Fontes, Amaral, Gomes, Bertarini, Brito-de-Sousa, Campi-Azevedo, Peruhype-Magalhães, Teixeira-Carvalho, Valim, Martins-Filho and da Mota https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Jardim-Santos, Gabriela Profírio
Schulte, Heidi Luise
Kurizky, Patricia Shu
Gomes, Ciro Martins
Nóbrega, Otávio Tolêdo
de Gois, Eliana Teles
de Carvalho, Maíra Rocha Machado
Martins, Francielle Pulccinelli
Nicola, André Moraes
de Albuquerque, Cleandro Pires
Espindola, Laila Salmen
Naves, Luciana Ansaneli
Soares, Alexandre Anderson de Sousa Munhoz
Albuquerque, Patrícia
Fontes, Wagner
do Amaral, Laurence Rodrigues
Gomes, Matheus de Souza
Bertarini, Pedro Luiz Lima
Brito-de-Sousa, Joaquim Pedro
Campi-Azevedo, Ana Carolina
Peruhype-Magalhães, Vanessa
Teixeira-Carvalho, Andrea
Valim, Valéria
Martins-Filho, Olindo Assis
da Mota, Licia Maria Henrique
Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title_full Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title_fullStr Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title_full_unstemmed Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title_short Unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe COVID-19 patients
title_sort unbalanced networks and disturbed kinetics of serum soluble mediators associated with distinct disease outcomes in severe covid-19 patients
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701840/
https://www.ncbi.nlm.nih.gov/pubmed/36451835
http://dx.doi.org/10.3389/fimmu.2022.1004023
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