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Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer
BACKGROUND: Copines‐1 (CPNE1) is a soluble membrane‐binding protein that includes two tandem C2 domains at the N‐terminus and a C terminal A domain. Importantly, it is associated with the prognosis of various tumors, but there are only a few studies regarding the role of CPNE1 in gastric cancer (GC)...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701855/ https://www.ncbi.nlm.nih.gov/pubmed/36310511 http://dx.doi.org/10.1002/jcla.24744 |
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author | Yang, Jun Wang, Yingjing Ge, Rong Jia, Xiupeng Ge, Congshan Cen, Youqing Pan, Deng |
author_facet | Yang, Jun Wang, Yingjing Ge, Rong Jia, Xiupeng Ge, Congshan Cen, Youqing Pan, Deng |
author_sort | Yang, Jun |
collection | PubMed |
description | BACKGROUND: Copines‐1 (CPNE1) is a soluble membrane‐binding protein that includes two tandem C2 domains at the N‐terminus and a C terminal A domain. Importantly, it is associated with the prognosis of various tumors, but there are only a few studies regarding the role of CPNE1 in gastric cancer (GC). This study aimed to explore the clinicopathological significance and prognostic potential of CPNE1 expression in GC. METHODS: Data from the TIMER2.0 and UALCAN were analyzed to assess CPNE1 mRNA levels in GC. The prognostic role of CPNE1 mRNA was examined via the Kaplan–Meier plotter. CPNE1 protein expression in tumor tissues was analyzed via immunohistochemistry of clinical samples from 99 GC patients. The relationship of CPNE1 expression with clinicopathological parameters and overall survival (OS) was evaluated using Cox proportional hazards regression models and Kaplan–Meier survival curves. RESULTS: Copines‐1 mRNA levels were higher in GC tissues than in adjacent normal tissue (ANT) (p < 0.05). Further, high CPNE1 mRNA expression indicated poor OS (p = 9.4 e‐10) and was significantly associated with first progression (FP) (p = 1.6 e‐06) and post‐progression survival (PPS) (p = 1.5 e‐12). In addition, CPNE1 protein expression was higher in GC tissues than in ANT (p < 0.0001). Moreover, CPNE1 high expression was significantly related to advanced tumor‐node‐metastasis (TNM) stage (p = 0.004), lymph node metastasis (p = 0.003), and vascular invasion (p = 0.001). Kaplan–Meier analysis showed that GC patients with high expression CPNE1 group had worse OS than low expression group (p = 0.003). Univariate analysis showed that age (hazard ratio [HR] = 1.992; 95% confidence interval [CI], 1.009–3.934; p = 0.047), advanced TNM stage (HR = 4.941; 95% CI, 2.052–11.897; p = 0.000), tumor invasion (HR = 3.472; 95% CI, 1.349–8.937; p = 0.010), lymph node metastasis (HR = 8.846; 95% CI, 2.708–28.897; p = 0.000), vascular invasion (HR = 3.237; 95% CI, 1.521–6.891; p = 0.002), nervous invasion (HR = 2.324; 95% CI, 1.205–4.479; p = 0.012), and CPNE1 expression (HR = 3.464; 95% CI, 1.440–8.334; p = 0.006) were correlated with OS. In the multivariate analysis, age (HR = 2.514; 95% CI, 1.264–4.999; p = 0.009), lymph node metastasis (HR = 8.441; 95% CI, 2.553–27.906; p < 0.05), and CPNE1 expression (HR = 2.549; 95% CI, 1.051–6.186; p = 0.039) were significant prognostic predictors for GC. CONCLUSIONS: Copines‐1 overexpression in GC is significantly associated with poor prognosis. Thus, CPNE1 levels may serve as a prognostic biomarker in GC patients. |
format | Online Article Text |
id | pubmed-9701855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97018552022-11-28 Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer Yang, Jun Wang, Yingjing Ge, Rong Jia, Xiupeng Ge, Congshan Cen, Youqing Pan, Deng J Clin Lab Anal Research Articles BACKGROUND: Copines‐1 (CPNE1) is a soluble membrane‐binding protein that includes two tandem C2 domains at the N‐terminus and a C terminal A domain. Importantly, it is associated with the prognosis of various tumors, but there are only a few studies regarding the role of CPNE1 in gastric cancer (GC). This study aimed to explore the clinicopathological significance and prognostic potential of CPNE1 expression in GC. METHODS: Data from the TIMER2.0 and UALCAN were analyzed to assess CPNE1 mRNA levels in GC. The prognostic role of CPNE1 mRNA was examined via the Kaplan–Meier plotter. CPNE1 protein expression in tumor tissues was analyzed via immunohistochemistry of clinical samples from 99 GC patients. The relationship of CPNE1 expression with clinicopathological parameters and overall survival (OS) was evaluated using Cox proportional hazards regression models and Kaplan–Meier survival curves. RESULTS: Copines‐1 mRNA levels were higher in GC tissues than in adjacent normal tissue (ANT) (p < 0.05). Further, high CPNE1 mRNA expression indicated poor OS (p = 9.4 e‐10) and was significantly associated with first progression (FP) (p = 1.6 e‐06) and post‐progression survival (PPS) (p = 1.5 e‐12). In addition, CPNE1 protein expression was higher in GC tissues than in ANT (p < 0.0001). Moreover, CPNE1 high expression was significantly related to advanced tumor‐node‐metastasis (TNM) stage (p = 0.004), lymph node metastasis (p = 0.003), and vascular invasion (p = 0.001). Kaplan–Meier analysis showed that GC patients with high expression CPNE1 group had worse OS than low expression group (p = 0.003). Univariate analysis showed that age (hazard ratio [HR] = 1.992; 95% confidence interval [CI], 1.009–3.934; p = 0.047), advanced TNM stage (HR = 4.941; 95% CI, 2.052–11.897; p = 0.000), tumor invasion (HR = 3.472; 95% CI, 1.349–8.937; p = 0.010), lymph node metastasis (HR = 8.846; 95% CI, 2.708–28.897; p = 0.000), vascular invasion (HR = 3.237; 95% CI, 1.521–6.891; p = 0.002), nervous invasion (HR = 2.324; 95% CI, 1.205–4.479; p = 0.012), and CPNE1 expression (HR = 3.464; 95% CI, 1.440–8.334; p = 0.006) were correlated with OS. In the multivariate analysis, age (HR = 2.514; 95% CI, 1.264–4.999; p = 0.009), lymph node metastasis (HR = 8.441; 95% CI, 2.553–27.906; p < 0.05), and CPNE1 expression (HR = 2.549; 95% CI, 1.051–6.186; p = 0.039) were significant prognostic predictors for GC. CONCLUSIONS: Copines‐1 overexpression in GC is significantly associated with poor prognosis. Thus, CPNE1 levels may serve as a prognostic biomarker in GC patients. John Wiley and Sons Inc. 2022-10-31 /pmc/articles/PMC9701855/ /pubmed/36310511 http://dx.doi.org/10.1002/jcla.24744 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Yang, Jun Wang, Yingjing Ge, Rong Jia, Xiupeng Ge, Congshan Cen, Youqing Pan, Deng Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title | Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title_full | Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title_fullStr | Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title_full_unstemmed | Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title_short | Overexpression of Copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
title_sort | overexpression of copines‐1 is associated with clinicopathological parameters and poor outcome in gastric cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701855/ https://www.ncbi.nlm.nih.gov/pubmed/36310511 http://dx.doi.org/10.1002/jcla.24744 |
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