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Identification of microRNA editing sites in three subtypes of leukemia

Leukemia is an aberrant hyper-proliferation of immature blood cells that do not form solid tumors. The transcriptomes of microRNAs (miRNAs) of leukemia have been intensively explored. However, miRNA editing of leukemia has not been extensively studied. To identify miRNA editing patterns and explore...

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Autores principales: Xie, Wenping, Yang, Jun, Zhou, Nan, Ding, Hao, Zhou, Guangchen, Wu, Shuai, Guo, Shiyong, Li, Wanran, Zhang, Lei, Yang, Huaide, Mao, Chunyi, Zheng, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702332/
https://www.ncbi.nlm.nih.gov/pubmed/36452459
http://dx.doi.org/10.3389/fmolb.2022.1014288
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author Xie, Wenping
Yang, Jun
Zhou, Nan
Ding, Hao
Zhou, Guangchen
Wu, Shuai
Guo, Shiyong
Li, Wanran
Zhang, Lei
Yang, Huaide
Mao, Chunyi
Zheng, Yun
author_facet Xie, Wenping
Yang, Jun
Zhou, Nan
Ding, Hao
Zhou, Guangchen
Wu, Shuai
Guo, Shiyong
Li, Wanran
Zhang, Lei
Yang, Huaide
Mao, Chunyi
Zheng, Yun
author_sort Xie, Wenping
collection PubMed
description Leukemia is an aberrant hyper-proliferation of immature blood cells that do not form solid tumors. The transcriptomes of microRNAs (miRNAs) of leukemia have been intensively explored. However, miRNA editing of leukemia has not been extensively studied. To identify miRNA editing patterns and explore their functional relevance in leukemia, we analyzed 200 small RNA sequencing profiles of three subtypes of leukemia and identified hundreds of miRNA editing sites in three subtypes of leukemia. Then, we compared the editing levels of identified miRNA editing sites in leukemia and normal controls. Many miRNAs were differential edited in different subtypes of leukemia. We also found the editing levels of 3′-A editing sites of hsa-mir-21-5p and hsa-mir-155-5p decreased in chronic lymphocytic leukemia patients with radiation treatments. By integrating PAR-CLIP sequencing profiles, we predicted the targets of original and edited miRNAs. One of the edited miRNA, hsa-let-7b_5c, with an additional cytosine at 5′ end of hsa-let-7b-5p, potentially targeted VBP1 and CTDSP1. CTDSP1 was significantly downregulated in T-ALL compared to normal controls, which might be originated from the hyperediting of hsa-let-7b-5p in T-ALL. Our study provides a comprehensive view of miRNA editing in three different subtypes of leukemia.
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spelling pubmed-97023322022-11-29 Identification of microRNA editing sites in three subtypes of leukemia Xie, Wenping Yang, Jun Zhou, Nan Ding, Hao Zhou, Guangchen Wu, Shuai Guo, Shiyong Li, Wanran Zhang, Lei Yang, Huaide Mao, Chunyi Zheng, Yun Front Mol Biosci Molecular Biosciences Leukemia is an aberrant hyper-proliferation of immature blood cells that do not form solid tumors. The transcriptomes of microRNAs (miRNAs) of leukemia have been intensively explored. However, miRNA editing of leukemia has not been extensively studied. To identify miRNA editing patterns and explore their functional relevance in leukemia, we analyzed 200 small RNA sequencing profiles of three subtypes of leukemia and identified hundreds of miRNA editing sites in three subtypes of leukemia. Then, we compared the editing levels of identified miRNA editing sites in leukemia and normal controls. Many miRNAs were differential edited in different subtypes of leukemia. We also found the editing levels of 3′-A editing sites of hsa-mir-21-5p and hsa-mir-155-5p decreased in chronic lymphocytic leukemia patients with radiation treatments. By integrating PAR-CLIP sequencing profiles, we predicted the targets of original and edited miRNAs. One of the edited miRNA, hsa-let-7b_5c, with an additional cytosine at 5′ end of hsa-let-7b-5p, potentially targeted VBP1 and CTDSP1. CTDSP1 was significantly downregulated in T-ALL compared to normal controls, which might be originated from the hyperediting of hsa-let-7b-5p in T-ALL. Our study provides a comprehensive view of miRNA editing in three different subtypes of leukemia. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9702332/ /pubmed/36452459 http://dx.doi.org/10.3389/fmolb.2022.1014288 Text en Copyright © 2022 Xie, Yang, Zhou, Ding, Zhou, Wu, Guo, Li, Zhang, Yang, Mao and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Xie, Wenping
Yang, Jun
Zhou, Nan
Ding, Hao
Zhou, Guangchen
Wu, Shuai
Guo, Shiyong
Li, Wanran
Zhang, Lei
Yang, Huaide
Mao, Chunyi
Zheng, Yun
Identification of microRNA editing sites in three subtypes of leukemia
title Identification of microRNA editing sites in three subtypes of leukemia
title_full Identification of microRNA editing sites in three subtypes of leukemia
title_fullStr Identification of microRNA editing sites in three subtypes of leukemia
title_full_unstemmed Identification of microRNA editing sites in three subtypes of leukemia
title_short Identification of microRNA editing sites in three subtypes of leukemia
title_sort identification of microrna editing sites in three subtypes of leukemia
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702332/
https://www.ncbi.nlm.nih.gov/pubmed/36452459
http://dx.doi.org/10.3389/fmolb.2022.1014288
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