Cargando…

Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice

Escherichia coli is one of the most common bacterial pathogens in cattle. Prophylactic vaccines are considered promising strategies with the potential to reduce the incidence of colibacillosis. Some studies suggested that bacterial ghosts may serve as a novel approach for preventing bacterial infect...

Descripción completa

Detalles Bibliográficos
Autores principales: Mu, Jing, Lei, Lei, Zheng, Yingce, Li, Ding, Li, Jie, Fu, Yunwei, Wang, Guanbo, Liu, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702981/
https://www.ncbi.nlm.nih.gov/pubmed/36451816
http://dx.doi.org/10.3389/fimmu.2022.1008131
_version_ 1784839763658801152
author Mu, Jing
Lei, Lei
Zheng, Yingce
Li, Ding
Li, Jie
Fu, Yunwei
Wang, Guanbo
Liu, Yun
author_facet Mu, Jing
Lei, Lei
Zheng, Yingce
Li, Ding
Li, Jie
Fu, Yunwei
Wang, Guanbo
Liu, Yun
author_sort Mu, Jing
collection PubMed
description Escherichia coli is one of the most common bacterial pathogens in cattle. Prophylactic vaccines are considered promising strategies with the potential to reduce the incidence of colibacillosis. Some studies suggested that bacterial ghosts may serve as a novel approach for preventing bacterial infections. However, the roles of administration route on vaccine immunogenicity and efficacy have not been investigated. In this study, the efficacy of vaccination via different immune routes in generating humoral and cellular immune response was compared through subcutaneous (SC), intramuscular (IM), and oral (O) administration in female BALB/c mice with bacterial ghosts prepared using wild type Escherichia coli isolates CE9, while phosphate buffer saline (PBS) and inactivated vaccines containing aluminum adjuvants (Killed) were used as control. Our results showed that the plasmid pBV220-E-aa-SNA containing E. coli was efficiently cleaved at 42°C with 94.8% positive ratio as assessed by colony counts. Transmission electron microscopy (TEM) confirmed bacteria retained intact surface structure while devoid of cytoplasmic component. We found that total IgG titers in killed, IM and SC groups showed significant increase on 7, 14, 21 and 28 days post-immunization. The IgA level of the IM group was higher than that of all other groups on the 28th day. Meanwhile, four experimental groups showed a significant difference in IgA levels compared with PBS control. In the IM group, an increase in the relative percentages of CD3+CD4+ T cells was accompanied by an increase in the relative percentages of splenic CD3+CD8+ T cells. In comparison with the inactivated vaccine, intramuscular CE9 ghosts immunization elicited higher levels of IL-1β, IL-2, IL-6 and IL-12. Subcutaneous and intramuscular immunizations were significantly associated with improved survival in comparison with oral route, traditional vaccine and the control. Pathologic assessment revealed that less severe tissue damage and inflammation were found in lung, kidney, and intestine of IM group compared with other groups. The results above demonstrate that immunization of Escherichia coli CE9 ghosts via intramuscular injection elicits a more robust antigen-specific immune response in mice to prevent the Escherichia coli infection.
format Online
Article
Text
id pubmed-9702981
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97029812022-11-29 Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice Mu, Jing Lei, Lei Zheng, Yingce Li, Ding Li, Jie Fu, Yunwei Wang, Guanbo Liu, Yun Front Immunol Immunology Escherichia coli is one of the most common bacterial pathogens in cattle. Prophylactic vaccines are considered promising strategies with the potential to reduce the incidence of colibacillosis. Some studies suggested that bacterial ghosts may serve as a novel approach for preventing bacterial infections. However, the roles of administration route on vaccine immunogenicity and efficacy have not been investigated. In this study, the efficacy of vaccination via different immune routes in generating humoral and cellular immune response was compared through subcutaneous (SC), intramuscular (IM), and oral (O) administration in female BALB/c mice with bacterial ghosts prepared using wild type Escherichia coli isolates CE9, while phosphate buffer saline (PBS) and inactivated vaccines containing aluminum adjuvants (Killed) were used as control. Our results showed that the plasmid pBV220-E-aa-SNA containing E. coli was efficiently cleaved at 42°C with 94.8% positive ratio as assessed by colony counts. Transmission electron microscopy (TEM) confirmed bacteria retained intact surface structure while devoid of cytoplasmic component. We found that total IgG titers in killed, IM and SC groups showed significant increase on 7, 14, 21 and 28 days post-immunization. The IgA level of the IM group was higher than that of all other groups on the 28th day. Meanwhile, four experimental groups showed a significant difference in IgA levels compared with PBS control. In the IM group, an increase in the relative percentages of CD3+CD4+ T cells was accompanied by an increase in the relative percentages of splenic CD3+CD8+ T cells. In comparison with the inactivated vaccine, intramuscular CE9 ghosts immunization elicited higher levels of IL-1β, IL-2, IL-6 and IL-12. Subcutaneous and intramuscular immunizations were significantly associated with improved survival in comparison with oral route, traditional vaccine and the control. Pathologic assessment revealed that less severe tissue damage and inflammation were found in lung, kidney, and intestine of IM group compared with other groups. The results above demonstrate that immunization of Escherichia coli CE9 ghosts via intramuscular injection elicits a more robust antigen-specific immune response in mice to prevent the Escherichia coli infection. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9702981/ /pubmed/36451816 http://dx.doi.org/10.3389/fimmu.2022.1008131 Text en Copyright © 2022 Mu, Lei, Zheng, Li, Li, Fu, Wang and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mu, Jing
Lei, Lei
Zheng, Yingce
Li, Ding
Li, Jie
Fu, Yunwei
Wang, Guanbo
Liu, Yun
Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title_full Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title_fullStr Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title_full_unstemmed Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title_short Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
title_sort comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic escherichia coli bacterial ghost vaccine in mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702981/
https://www.ncbi.nlm.nih.gov/pubmed/36451816
http://dx.doi.org/10.3389/fimmu.2022.1008131
work_keys_str_mv AT mujing comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT leilei comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT zhengyingce comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT liding comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT lijie comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT fuyunwei comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT wangguanbo comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice
AT liuyun comparativestudyofsubcutaneousintramuscularandoraladministrationofbovinepathogenicescherichiacolibacterialghostvaccineinmice