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Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome

Exposure to glyphosate (GLY), a commonly used herbicide, is supported by urinary detection and associated with shortened gestation in women. This study tested the hypothesis that chronic low-dose pre-conceptional GLY exposure would affect maternal ovarian function mid- and post-gestation. Mice (C57B...

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Autores principales: Novbatova, Gulnara, Timme, Kelsey, Severin, Andrew, Sayadi, Maryam, Keating, Aileen F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702999/
https://www.ncbi.nlm.nih.gov/pubmed/36173347
http://dx.doi.org/10.1093/toxsci/kfac098
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author Novbatova, Gulnara
Timme, Kelsey
Severin, Andrew
Sayadi, Maryam
Keating, Aileen F
author_facet Novbatova, Gulnara
Timme, Kelsey
Severin, Andrew
Sayadi, Maryam
Keating, Aileen F
author_sort Novbatova, Gulnara
collection PubMed
description Exposure to glyphosate (GLY), a commonly used herbicide, is supported by urinary detection and associated with shortened gestation in women. This study tested the hypothesis that chronic low-dose pre-conceptional GLY exposure would affect maternal ovarian function mid- and post-gestation. Mice (C57BL/6; n = 40) were exposed per os to saline vehicle control (CT; n = 20) or GLY (2 mg/kg; n = 20) daily for 10 weeks starting at 7 weeks of age. Post-exposure, females were impregnated and euthanized at gestation day 14 (GD14) or post-weaning (PW). Pregnancy success was reduced from 75% to 55% by GLY exposure. No treatment effect (p > .05) on body weight, maternal serum 17β-estradiol, or litter size was noted. Ovarian weight was unaffected or reduced (p < .05) by GLY in GD14 and PW dams, respectively. Exposure to GLY decreased (p < .05) PW ovarian secondary follicle number with no other follicle composition impacts. Protein abundance analysis by LC-MS/MS identified that GLY altered (p < .05) 26 ovarian and 41 hepatic proteins in GD14 dams and 39 hepatic proteins in PW dams. In GD14 dams, GLY increased ovarian protein abundance of SEC16A (p < .05; 29-fold) and hepatic RPS27L and GM4952 (p < .05; ∼4-fold). In both GD14 and PW dams, GLY exposure increased (p < .05) hepatic RPS4 and decreased (p < .05) ECHDC3. Pathway analysis using DAVID identified 10 GLY hepatic pathway targets with FDR ≤ 0.07 in GD14 dams.
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spelling pubmed-97029992022-12-24 Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome Novbatova, Gulnara Timme, Kelsey Severin, Andrew Sayadi, Maryam Keating, Aileen F Toxicol Sci Endocrine Toxicology Exposure to glyphosate (GLY), a commonly used herbicide, is supported by urinary detection and associated with shortened gestation in women. This study tested the hypothesis that chronic low-dose pre-conceptional GLY exposure would affect maternal ovarian function mid- and post-gestation. Mice (C57BL/6; n = 40) were exposed per os to saline vehicle control (CT; n = 20) or GLY (2 mg/kg; n = 20) daily for 10 weeks starting at 7 weeks of age. Post-exposure, females were impregnated and euthanized at gestation day 14 (GD14) or post-weaning (PW). Pregnancy success was reduced from 75% to 55% by GLY exposure. No treatment effect (p > .05) on body weight, maternal serum 17β-estradiol, or litter size was noted. Ovarian weight was unaffected or reduced (p < .05) by GLY in GD14 and PW dams, respectively. Exposure to GLY decreased (p < .05) PW ovarian secondary follicle number with no other follicle composition impacts. Protein abundance analysis by LC-MS/MS identified that GLY altered (p < .05) 26 ovarian and 41 hepatic proteins in GD14 dams and 39 hepatic proteins in PW dams. In GD14 dams, GLY increased ovarian protein abundance of SEC16A (p < .05; 29-fold) and hepatic RPS27L and GM4952 (p < .05; ∼4-fold). In both GD14 and PW dams, GLY exposure increased (p < .05) hepatic RPS4 and decreased (p < .05) ECHDC3. Pathway analysis using DAVID identified 10 GLY hepatic pathway targets with FDR ≤ 0.07 in GD14 dams. Oxford University Press 2022-09-29 /pmc/articles/PMC9702999/ /pubmed/36173347 http://dx.doi.org/10.1093/toxsci/kfac098 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the Society of Toxicology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Endocrine Toxicology
Novbatova, Gulnara
Timme, Kelsey
Severin, Andrew
Sayadi, Maryam
Keating, Aileen F
Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title_full Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title_fullStr Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title_full_unstemmed Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title_short Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome
title_sort pre-conceptional exposure to glyphosate affects the maternal hepatic and ovarian proteome
topic Endocrine Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702999/
https://www.ncbi.nlm.nih.gov/pubmed/36173347
http://dx.doi.org/10.1093/toxsci/kfac098
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