Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhance...

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Detalles Bibliográficos
Autores principales: Correddu, Danilo, Helmy Aly, Sabrina, Di Nardo, Giovanna, Catucci, Gianluca, Prandi, Cristina, Blangetti, Marco, Bellomo, Chiara, Bonometti, Elisabetta, Viscardi, Guido, Gilardi, Gianfranco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9703296/
https://www.ncbi.nlm.nih.gov/pubmed/36505709
http://dx.doi.org/10.1039/d2ra06029a
Descripción
Sumario:Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.

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