Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhance...

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Autores principales: Correddu, Danilo, Helmy Aly, Sabrina, Di Nardo, Giovanna, Catucci, Gianluca, Prandi, Cristina, Blangetti, Marco, Bellomo, Chiara, Bonometti, Elisabetta, Viscardi, Guido, Gilardi, Gianfranco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9703296/
https://www.ncbi.nlm.nih.gov/pubmed/36505709
http://dx.doi.org/10.1039/d2ra06029a
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author Correddu, Danilo
Helmy Aly, Sabrina
Di Nardo, Giovanna
Catucci, Gianluca
Prandi, Cristina
Blangetti, Marco
Bellomo, Chiara
Bonometti, Elisabetta
Viscardi, Guido
Gilardi, Gianfranco
author_facet Correddu, Danilo
Helmy Aly, Sabrina
Di Nardo, Giovanna
Catucci, Gianluca
Prandi, Cristina
Blangetti, Marco
Bellomo, Chiara
Bonometti, Elisabetta
Viscardi, Guido
Gilardi, Gianfranco
author_sort Correddu, Danilo
collection PubMed
description Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.
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spelling pubmed-97032962022-12-08 Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives Correddu, Danilo Helmy Aly, Sabrina Di Nardo, Giovanna Catucci, Gianluca Prandi, Cristina Blangetti, Marco Bellomo, Chiara Bonometti, Elisabetta Viscardi, Guido Gilardi, Gianfranco RSC Adv Chemistry Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications. The Royal Society of Chemistry 2022-11-28 /pmc/articles/PMC9703296/ /pubmed/36505709 http://dx.doi.org/10.1039/d2ra06029a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Correddu, Danilo
Helmy Aly, Sabrina
Di Nardo, Giovanna
Catucci, Gianluca
Prandi, Cristina
Blangetti, Marco
Bellomo, Chiara
Bonometti, Elisabetta
Viscardi, Guido
Gilardi, Gianfranco
Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title_full Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title_fullStr Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title_full_unstemmed Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title_short Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
title_sort enhanced and specific epoxidation activity of p450 bm3 mutants for the production of high value terpene derivatives
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9703296/
https://www.ncbi.nlm.nih.gov/pubmed/36505709
http://dx.doi.org/10.1039/d2ra06029a
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