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Identification of a growth factor required for culturing specific fastidious oral bacteria
AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus homini...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704088/ https://www.ncbi.nlm.nih.gov/pubmed/36452178 http://dx.doi.org/10.1080/20002297.2022.2143651 |
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author | Murugkar, Pallavi Dimise, Eric Stewart, Eric Viala, Stéphane N. Clardy, Jon Dewhirst, Floyd E. Lewis, Kim |
author_facet | Murugkar, Pallavi Dimise, Eric Stewart, Eric Viala, Stéphane N. Clardy, Jon Dewhirst, Floyd E. Lewis, Kim |
author_sort | Murugkar, Pallavi |
collection | PubMed |
description | AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus hominis and Escherichia coli as helper strains. A deletion mutant library of E. coli was screened to determine genes involved in production of the growth factor. Compounds produced by the growth factor’s pathway were screened to see if they would stimulate growth of strain P. pasteri KLE1280. The genomes of species related to P. pasteri KLE1280 were screened for presence of the factor’s synthetic pathway. RESULTS: Analysis of the E. coli deletion mutant library and growth studies identified 1,2-dihydroxy-2-naphthoic acid (DHNA) and menaquinone-4 (MK4) as the growth factors. Strain P. pasteri KLE1280 was shown to lack five genes in the menaquinone synthesis pathway but to possess the two genes necessary to convert DHNA to menaquinone. Genome analysis found that 8 species in genera Porphyromonas and Tannerella lack five genes in the menaquinone synthesis pathway. CONCLUSIONS: Addition of DHNA to culture media allows isolation of strains of several oral species that are not recovered using standard media |
format | Online Article Text |
id | pubmed-9704088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-97040882022-11-29 Identification of a growth factor required for culturing specific fastidious oral bacteria Murugkar, Pallavi Dimise, Eric Stewart, Eric Viala, Stéphane N. Clardy, Jon Dewhirst, Floyd E. Lewis, Kim J Oral Microbiol Original Article AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus hominis and Escherichia coli as helper strains. A deletion mutant library of E. coli was screened to determine genes involved in production of the growth factor. Compounds produced by the growth factor’s pathway were screened to see if they would stimulate growth of strain P. pasteri KLE1280. The genomes of species related to P. pasteri KLE1280 were screened for presence of the factor’s synthetic pathway. RESULTS: Analysis of the E. coli deletion mutant library and growth studies identified 1,2-dihydroxy-2-naphthoic acid (DHNA) and menaquinone-4 (MK4) as the growth factors. Strain P. pasteri KLE1280 was shown to lack five genes in the menaquinone synthesis pathway but to possess the two genes necessary to convert DHNA to menaquinone. Genome analysis found that 8 species in genera Porphyromonas and Tannerella lack five genes in the menaquinone synthesis pathway. CONCLUSIONS: Addition of DHNA to culture media allows isolation of strains of several oral species that are not recovered using standard media Taylor & Francis 2022-11-25 /pmc/articles/PMC9704088/ /pubmed/36452178 http://dx.doi.org/10.1080/20002297.2022.2143651 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Murugkar, Pallavi Dimise, Eric Stewart, Eric Viala, Stéphane N. Clardy, Jon Dewhirst, Floyd E. Lewis, Kim Identification of a growth factor required for culturing specific fastidious oral bacteria |
title | Identification of a growth factor required for culturing specific fastidious oral bacteria |
title_full | Identification of a growth factor required for culturing specific fastidious oral bacteria |
title_fullStr | Identification of a growth factor required for culturing specific fastidious oral bacteria |
title_full_unstemmed | Identification of a growth factor required for culturing specific fastidious oral bacteria |
title_short | Identification of a growth factor required for culturing specific fastidious oral bacteria |
title_sort | identification of a growth factor required for culturing specific fastidious oral bacteria |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704088/ https://www.ncbi.nlm.nih.gov/pubmed/36452178 http://dx.doi.org/10.1080/20002297.2022.2143651 |
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