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Identification of a growth factor required for culturing specific fastidious oral bacteria

AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus homini...

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Autores principales: Murugkar, Pallavi, Dimise, Eric, Stewart, Eric, Viala, Stéphane N., Clardy, Jon, Dewhirst, Floyd E., Lewis, Kim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704088/
https://www.ncbi.nlm.nih.gov/pubmed/36452178
http://dx.doi.org/10.1080/20002297.2022.2143651
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author Murugkar, Pallavi
Dimise, Eric
Stewart, Eric
Viala, Stéphane N.
Clardy, Jon
Dewhirst, Floyd E.
Lewis, Kim
author_facet Murugkar, Pallavi
Dimise, Eric
Stewart, Eric
Viala, Stéphane N.
Clardy, Jon
Dewhirst, Floyd E.
Lewis, Kim
author_sort Murugkar, Pallavi
collection PubMed
description AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus hominis and Escherichia coli as helper strains. A deletion mutant library of E. coli was screened to determine genes involved in production of the growth factor. Compounds produced by the growth factor’s pathway were screened to see if they would stimulate growth of strain P. pasteri KLE1280. The genomes of species related to P. pasteri KLE1280 were screened for presence of the factor’s synthetic pathway. RESULTS: Analysis of the E. coli deletion mutant library and growth studies identified 1,2-dihydroxy-2-naphthoic acid (DHNA) and menaquinone-4 (MK4) as the growth factors. Strain P. pasteri KLE1280 was shown to lack five genes in the menaquinone synthesis pathway but to possess the two genes necessary to convert DHNA to menaquinone. Genome analysis found that 8 species in genera Porphyromonas and Tannerella lack five genes in the menaquinone synthesis pathway. CONCLUSIONS: Addition of DHNA to culture media allows isolation of strains of several oral species that are not recovered using standard media
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spelling pubmed-97040882022-11-29 Identification of a growth factor required for culturing specific fastidious oral bacteria Murugkar, Pallavi Dimise, Eric Stewart, Eric Viala, Stéphane N. Clardy, Jon Dewhirst, Floyd E. Lewis, Kim J Oral Microbiol Original Article AIMS: The aim of this research was to isolate oral bacteria that are dependent for growth on adjacent bacteria producing a required growth factor and to identify the chemical structure of the growth factor. METHODS: Porphyromonas pasteri strain KLE1280, could be cultivated with Staphylococcus hominis and Escherichia coli as helper strains. A deletion mutant library of E. coli was screened to determine genes involved in production of the growth factor. Compounds produced by the growth factor’s pathway were screened to see if they would stimulate growth of strain P. pasteri KLE1280. The genomes of species related to P. pasteri KLE1280 were screened for presence of the factor’s synthetic pathway. RESULTS: Analysis of the E. coli deletion mutant library and growth studies identified 1,2-dihydroxy-2-naphthoic acid (DHNA) and menaquinone-4 (MK4) as the growth factors. Strain P. pasteri KLE1280 was shown to lack five genes in the menaquinone synthesis pathway but to possess the two genes necessary to convert DHNA to menaquinone. Genome analysis found that 8 species in genera Porphyromonas and Tannerella lack five genes in the menaquinone synthesis pathway. CONCLUSIONS: Addition of DHNA to culture media allows isolation of strains of several oral species that are not recovered using standard media Taylor & Francis 2022-11-25 /pmc/articles/PMC9704088/ /pubmed/36452178 http://dx.doi.org/10.1080/20002297.2022.2143651 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Murugkar, Pallavi
Dimise, Eric
Stewart, Eric
Viala, Stéphane N.
Clardy, Jon
Dewhirst, Floyd E.
Lewis, Kim
Identification of a growth factor required for culturing specific fastidious oral bacteria
title Identification of a growth factor required for culturing specific fastidious oral bacteria
title_full Identification of a growth factor required for culturing specific fastidious oral bacteria
title_fullStr Identification of a growth factor required for culturing specific fastidious oral bacteria
title_full_unstemmed Identification of a growth factor required for culturing specific fastidious oral bacteria
title_short Identification of a growth factor required for culturing specific fastidious oral bacteria
title_sort identification of a growth factor required for culturing specific fastidious oral bacteria
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704088/
https://www.ncbi.nlm.nih.gov/pubmed/36452178
http://dx.doi.org/10.1080/20002297.2022.2143651
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