Cargando…
Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli
New antimicrobials need to be discovered to fight the advance of multidrug-resistant pathogens. A promising approach is the screening for antimicrobial agents naturally produced by living organisms. As an alternative to studying the native producer, it is possible to use genetically tractable microb...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704530/ https://www.ncbi.nlm.nih.gov/pubmed/36196987 http://dx.doi.org/10.1042/BST20220218 |
_version_ | 1784840074571022336 |
---|---|
author | Iacovelli, Riccardo Sokolova, Nika Haslinger, Kristina |
author_facet | Iacovelli, Riccardo Sokolova, Nika Haslinger, Kristina |
author_sort | Iacovelli, Riccardo |
collection | PubMed |
description | New antimicrobials need to be discovered to fight the advance of multidrug-resistant pathogens. A promising approach is the screening for antimicrobial agents naturally produced by living organisms. As an alternative to studying the native producer, it is possible to use genetically tractable microbes as heterologous hosts to aid the discovery process, facilitate product diversification through genetic engineering, and ultimately enable environmentally friendly production. In this mini-review, we summarize the literature from 2017 to 2022 on the application of Escherichia coli and E. coli-based platforms as versatile and powerful systems for the discovery, characterization, and sustainable production of antimicrobials. We highlight recent developments in high-throughput screening methods and genetic engineering approaches that build on the strengths of E. coli as an expression host and that led to the production of antimicrobial compounds. In the last section, we briefly discuss new techniques that have not been applied to discover or engineer antimicrobials yet, but that may be useful for this application in the future. |
format | Online Article Text |
id | pubmed-9704530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97045302022-12-06 Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli Iacovelli, Riccardo Sokolova, Nika Haslinger, Kristina Biochem Soc Trans Review Articles New antimicrobials need to be discovered to fight the advance of multidrug-resistant pathogens. A promising approach is the screening for antimicrobial agents naturally produced by living organisms. As an alternative to studying the native producer, it is possible to use genetically tractable microbes as heterologous hosts to aid the discovery process, facilitate product diversification through genetic engineering, and ultimately enable environmentally friendly production. In this mini-review, we summarize the literature from 2017 to 2022 on the application of Escherichia coli and E. coli-based platforms as versatile and powerful systems for the discovery, characterization, and sustainable production of antimicrobials. We highlight recent developments in high-throughput screening methods and genetic engineering approaches that build on the strengths of E. coli as an expression host and that led to the production of antimicrobial compounds. In the last section, we briefly discuss new techniques that have not been applied to discover or engineer antimicrobials yet, but that may be useful for this application in the future. Portland Press Ltd. 2022-10-31 2022-10-05 /pmc/articles/PMC9704530/ /pubmed/36196987 http://dx.doi.org/10.1042/BST20220218 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Review Articles Iacovelli, Riccardo Sokolova, Nika Haslinger, Kristina Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title | Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title_full | Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title_fullStr | Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title_full_unstemmed | Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title_short | Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli |
title_sort | striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in escherichia coli |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704530/ https://www.ncbi.nlm.nih.gov/pubmed/36196987 http://dx.doi.org/10.1042/BST20220218 |
work_keys_str_mv | AT iacovelliriccardo strivingforsustainablebiosynthesisdiscoverydiversificationandproductionofantimicrobialdrugsinescherichiacoli AT sokolovanika strivingforsustainablebiosynthesisdiscoverydiversificationandproductionofantimicrobialdrugsinescherichiacoli AT haslingerkristina strivingforsustainablebiosynthesisdiscoverydiversificationandproductionofantimicrobialdrugsinescherichiacoli |