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Strength in numbers: effect of protein crowding on the shape of cell membranes
Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704533/ https://www.ncbi.nlm.nih.gov/pubmed/36214373 http://dx.doi.org/10.1042/BST20210883 |
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author | Ruhoff, Victoria Thusgaard Moreno-Pescador, Guillermo Pezeshkian, Weria Bendix, Poul Martin |
author_facet | Ruhoff, Victoria Thusgaard Moreno-Pescador, Guillermo Pezeshkian, Weria Bendix, Poul Martin |
author_sort | Ruhoff, Victoria Thusgaard |
collection | PubMed |
description | Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown to be involved in overcoming the energy barrier associated with membrane bending. The reported mechanisms behind membrane bending have been largely concerned with structural protein features, however, in the last decade, reports on the ability of densely packed proteins to bend membranes by protein–protein crowding, have challenged prevailing mechanistic views. Crowding has now been shown to generate spontaneous vesicle formation and tubular morphologies on cell- and model membranes, demonstrating crowding as a relevant player involved in the bending of membranes. Still, current research is largely based on unnatural overexpression of proteins in non-native domains, and together with efforts in modeling, this has led to questioning the in vivo impact of crowding. In this review, we examine this previously overlooked mechanism by summarizing recent advances in the understanding of protein–protein crowding and its prevalence in cellular membrane-shaping processes. |
format | Online Article Text |
id | pubmed-9704533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97045332022-12-06 Strength in numbers: effect of protein crowding on the shape of cell membranes Ruhoff, Victoria Thusgaard Moreno-Pescador, Guillermo Pezeshkian, Weria Bendix, Poul Martin Biochem Soc Trans Review Articles Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown to be involved in overcoming the energy barrier associated with membrane bending. The reported mechanisms behind membrane bending have been largely concerned with structural protein features, however, in the last decade, reports on the ability of densely packed proteins to bend membranes by protein–protein crowding, have challenged prevailing mechanistic views. Crowding has now been shown to generate spontaneous vesicle formation and tubular morphologies on cell- and model membranes, demonstrating crowding as a relevant player involved in the bending of membranes. Still, current research is largely based on unnatural overexpression of proteins in non-native domains, and together with efforts in modeling, this has led to questioning the in vivo impact of crowding. In this review, we examine this previously overlooked mechanism by summarizing recent advances in the understanding of protein–protein crowding and its prevalence in cellular membrane-shaping processes. Portland Press Ltd. 2022-10-31 2022-10-10 /pmc/articles/PMC9704533/ /pubmed/36214373 http://dx.doi.org/10.1042/BST20210883 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Articles Ruhoff, Victoria Thusgaard Moreno-Pescador, Guillermo Pezeshkian, Weria Bendix, Poul Martin Strength in numbers: effect of protein crowding on the shape of cell membranes |
title | Strength in numbers: effect of protein crowding on the shape of cell membranes |
title_full | Strength in numbers: effect of protein crowding on the shape of cell membranes |
title_fullStr | Strength in numbers: effect of protein crowding on the shape of cell membranes |
title_full_unstemmed | Strength in numbers: effect of protein crowding on the shape of cell membranes |
title_short | Strength in numbers: effect of protein crowding on the shape of cell membranes |
title_sort | strength in numbers: effect of protein crowding on the shape of cell membranes |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704533/ https://www.ncbi.nlm.nih.gov/pubmed/36214373 http://dx.doi.org/10.1042/BST20210883 |
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