Cargando…

Strength in numbers: effect of protein crowding on the shape of cell membranes

Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown t...

Descripción completa

Detalles Bibliográficos
Autores principales: Ruhoff, Victoria Thusgaard, Moreno-Pescador, Guillermo, Pezeshkian, Weria, Bendix, Poul Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704533/
https://www.ncbi.nlm.nih.gov/pubmed/36214373
http://dx.doi.org/10.1042/BST20210883
_version_ 1784840075378425856
author Ruhoff, Victoria Thusgaard
Moreno-Pescador, Guillermo
Pezeshkian, Weria
Bendix, Poul Martin
author_facet Ruhoff, Victoria Thusgaard
Moreno-Pescador, Guillermo
Pezeshkian, Weria
Bendix, Poul Martin
author_sort Ruhoff, Victoria Thusgaard
collection PubMed
description Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown to be involved in overcoming the energy barrier associated with membrane bending. The reported mechanisms behind membrane bending have been largely concerned with structural protein features, however, in the last decade, reports on the ability of densely packed proteins to bend membranes by protein–protein crowding, have challenged prevailing mechanistic views. Crowding has now been shown to generate spontaneous vesicle formation and tubular morphologies on cell- and model membranes, demonstrating crowding as a relevant player involved in the bending of membranes. Still, current research is largely based on unnatural overexpression of proteins in non-native domains, and together with efforts in modeling, this has led to questioning the in vivo impact of crowding. In this review, we examine this previously overlooked mechanism by summarizing recent advances in the understanding of protein–protein crowding and its prevalence in cellular membrane-shaping processes.
format Online
Article
Text
id pubmed-9704533
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-97045332022-12-06 Strength in numbers: effect of protein crowding on the shape of cell membranes Ruhoff, Victoria Thusgaard Moreno-Pescador, Guillermo Pezeshkian, Weria Bendix, Poul Martin Biochem Soc Trans Review Articles Continuous reshaping of the plasma membrane into pleomorphic shapes is critical for a plethora of cellular functions. How the cell carries out this enigmatic control of membrane remodeling has remained an active research field for decades and several molecular and biophysical mechanisms have shown to be involved in overcoming the energy barrier associated with membrane bending. The reported mechanisms behind membrane bending have been largely concerned with structural protein features, however, in the last decade, reports on the ability of densely packed proteins to bend membranes by protein–protein crowding, have challenged prevailing mechanistic views. Crowding has now been shown to generate spontaneous vesicle formation and tubular morphologies on cell- and model membranes, demonstrating crowding as a relevant player involved in the bending of membranes. Still, current research is largely based on unnatural overexpression of proteins in non-native domains, and together with efforts in modeling, this has led to questioning the in vivo impact of crowding. In this review, we examine this previously overlooked mechanism by summarizing recent advances in the understanding of protein–protein crowding and its prevalence in cellular membrane-shaping processes. Portland Press Ltd. 2022-10-31 2022-10-10 /pmc/articles/PMC9704533/ /pubmed/36214373 http://dx.doi.org/10.1042/BST20210883 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Articles
Ruhoff, Victoria Thusgaard
Moreno-Pescador, Guillermo
Pezeshkian, Weria
Bendix, Poul Martin
Strength in numbers: effect of protein crowding on the shape of cell membranes
title Strength in numbers: effect of protein crowding on the shape of cell membranes
title_full Strength in numbers: effect of protein crowding on the shape of cell membranes
title_fullStr Strength in numbers: effect of protein crowding on the shape of cell membranes
title_full_unstemmed Strength in numbers: effect of protein crowding on the shape of cell membranes
title_short Strength in numbers: effect of protein crowding on the shape of cell membranes
title_sort strength in numbers: effect of protein crowding on the shape of cell membranes
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704533/
https://www.ncbi.nlm.nih.gov/pubmed/36214373
http://dx.doi.org/10.1042/BST20210883
work_keys_str_mv AT ruhoffvictoriathusgaard strengthinnumberseffectofproteincrowdingontheshapeofcellmembranes
AT morenopescadorguillermo strengthinnumberseffectofproteincrowdingontheshapeofcellmembranes
AT pezeshkianweria strengthinnumberseffectofproteincrowdingontheshapeofcellmembranes
AT bendixpoulmartin strengthinnumberseffectofproteincrowdingontheshapeofcellmembranes