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3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

The use of hydrogel as a filling medium to recombine dispersed microencapsulated cells to form an embedded gel-cell microcapsule complex is a new idea based on bottom-up tissue construction, which is benefit for cell distribution and of great significance for tissue construction research in vitro. I...

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Autores principales: Long, Ruimin, Shi, Linrong, He, Peng, Tian, Jumei, Wang, Shibin, Zheng, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704540/
https://www.ncbi.nlm.nih.gov/pubmed/36452208
http://dx.doi.org/10.3389/fbioe.2022.1056652
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author Long, Ruimin
Shi, Linrong
He, Peng
Tian, Jumei
Wang, Shibin
Zheng, Jun
author_facet Long, Ruimin
Shi, Linrong
He, Peng
Tian, Jumei
Wang, Shibin
Zheng, Jun
author_sort Long, Ruimin
collection PubMed
description The use of hydrogel as a filling medium to recombine dispersed microencapsulated cells to form an embedded gel-cell microcapsule complex is a new idea based on bottom-up tissue construction, which is benefit for cell distribution and of great significance for tissue construction research in vitro. In this experiment, sodium alginate and chitosan were used as the main materials, rat normal liver cell BRL-3A was used as the model cell to prepare “artificial cells”. Silkworm pupa was used as raw material to extract silk fibroin solution, which was prepared by ultrasound to be the silk fibroin gel; silk fibroin hydrogel-microencapsulated hepatocyte embedded complex was then prepared by using silk fibroin gel as filling medium; the complex was cultured under three modes (static, shaking, and 3D microgravity), and the tissue forming ability of rat hepatocytes was investigated. The results showed that the microgravity culture condition can enhance the cell proliferation and promote the formation of cell colonies in the microcapsules; silk fibroin can form an embedded gel-cell microcapsule complex with microencapsulated cells, which provided mechanical support for the structure of the composite. We hope that this bottom-up construction system will have potential applications in the fields of cell culture and tissue construction.
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spelling pubmed-97045402022-11-29 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs Long, Ruimin Shi, Linrong He, Peng Tian, Jumei Wang, Shibin Zheng, Jun Front Bioeng Biotechnol Bioengineering and Biotechnology The use of hydrogel as a filling medium to recombine dispersed microencapsulated cells to form an embedded gel-cell microcapsule complex is a new idea based on bottom-up tissue construction, which is benefit for cell distribution and of great significance for tissue construction research in vitro. In this experiment, sodium alginate and chitosan were used as the main materials, rat normal liver cell BRL-3A was used as the model cell to prepare “artificial cells”. Silkworm pupa was used as raw material to extract silk fibroin solution, which was prepared by ultrasound to be the silk fibroin gel; silk fibroin hydrogel-microencapsulated hepatocyte embedded complex was then prepared by using silk fibroin gel as filling medium; the complex was cultured under three modes (static, shaking, and 3D microgravity), and the tissue forming ability of rat hepatocytes was investigated. The results showed that the microgravity culture condition can enhance the cell proliferation and promote the formation of cell colonies in the microcapsules; silk fibroin can form an embedded gel-cell microcapsule complex with microencapsulated cells, which provided mechanical support for the structure of the composite. We hope that this bottom-up construction system will have potential applications in the fields of cell culture and tissue construction. Frontiers Media S.A. 2022-11-14 /pmc/articles/PMC9704540/ /pubmed/36452208 http://dx.doi.org/10.3389/fbioe.2022.1056652 Text en Copyright © 2022 Long, Shi, He, Tian, Wang and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Long, Ruimin
Shi, Linrong
He, Peng
Tian, Jumei
Wang, Shibin
Zheng, Jun
3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title_full 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title_fullStr 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title_full_unstemmed 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title_short 3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
title_sort 3d cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704540/
https://www.ncbi.nlm.nih.gov/pubmed/36452208
http://dx.doi.org/10.3389/fbioe.2022.1056652
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