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Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model

The arrhythmic substrate of ventricular tachycardias in many structural heart diseases is located in the epicardium, often resulting in poor outcomes with currently available therapies. Cardiosphere-derived cells (CDCs) have been shown to modify myocardial scarring. A total of 19 Large White pigs we...

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Autores principales: Carta-Bergaz, Alejandro, Ríos-Muñoz, Gonzalo R., Crisóstomo, Verónica, Sánchez-Margallo, Francisco M., Ledesma-Carbayo, María J., Bermejo-Thomas, Javier, Fernández-Avilés, Francisco, Arenal-Maíz, Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705343/
https://www.ncbi.nlm.nih.gov/pubmed/36457311
http://dx.doi.org/10.3389/fphys.2022.1041348
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author Carta-Bergaz, Alejandro
Ríos-Muñoz, Gonzalo R.
Crisóstomo, Verónica
Sánchez-Margallo, Francisco M.
Ledesma-Carbayo, María J.
Bermejo-Thomas, Javier
Fernández-Avilés, Francisco
Arenal-Maíz, Ángel
author_facet Carta-Bergaz, Alejandro
Ríos-Muñoz, Gonzalo R.
Crisóstomo, Verónica
Sánchez-Margallo, Francisco M.
Ledesma-Carbayo, María J.
Bermejo-Thomas, Javier
Fernández-Avilés, Francisco
Arenal-Maíz, Ángel
author_sort Carta-Bergaz, Alejandro
collection PubMed
description The arrhythmic substrate of ventricular tachycardias in many structural heart diseases is located in the epicardium, often resulting in poor outcomes with currently available therapies. Cardiosphere-derived cells (CDCs) have been shown to modify myocardial scarring. A total of 19 Large White pigs were infarcted by occlusion of the mid-left anterior descending coronary artery for 150 min. Baseline cardiac magnetic resonance (CMR) imaging with late gadolinium enhancement sequences was obtained 4 weeks post-infarction and pigs were randomized to a treatment group (intrapericardial administration of 300,000 allogeneic CDCs/kg), (n = 10) and to a control group (n = 9). A second CMR and high-density endocardial electroanatomical mapping were performed at 16 weeks post-infarction. After the electrophysiological study, pigs were sacrificed and epicardial optical mapping and histological studies of the heterogeneous tissue of the endocardial and epicardial scars were performed. In comparison with control conditions, intrapericardial CDCs reduced the growth of epicardial dense scar and epicardial electrical heterogeneity. The relative differences in conduction velocity and action potential duration between healthy myocardium and heterogeneous tissue were significantly smaller in the CDC-treated group than in the control group. The lower electrical heterogeneity coincides with heterogeneous tissue with less fibrosis, better cardiomyocyte viability, and a greater quantity and better polarity of connexin 43. At the endocardial level, no differences were detected between groups. Intrapericardial CDCs produce anatomical and functional changes in the epicardial arrhythmic substrate, which could have an anti-arrhythmic effect.
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spelling pubmed-97053432022-11-30 Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model Carta-Bergaz, Alejandro Ríos-Muñoz, Gonzalo R. Crisóstomo, Verónica Sánchez-Margallo, Francisco M. Ledesma-Carbayo, María J. Bermejo-Thomas, Javier Fernández-Avilés, Francisco Arenal-Maíz, Ángel Front Physiol Physiology The arrhythmic substrate of ventricular tachycardias in many structural heart diseases is located in the epicardium, often resulting in poor outcomes with currently available therapies. Cardiosphere-derived cells (CDCs) have been shown to modify myocardial scarring. A total of 19 Large White pigs were infarcted by occlusion of the mid-left anterior descending coronary artery for 150 min. Baseline cardiac magnetic resonance (CMR) imaging with late gadolinium enhancement sequences was obtained 4 weeks post-infarction and pigs were randomized to a treatment group (intrapericardial administration of 300,000 allogeneic CDCs/kg), (n = 10) and to a control group (n = 9). A second CMR and high-density endocardial electroanatomical mapping were performed at 16 weeks post-infarction. After the electrophysiological study, pigs were sacrificed and epicardial optical mapping and histological studies of the heterogeneous tissue of the endocardial and epicardial scars were performed. In comparison with control conditions, intrapericardial CDCs reduced the growth of epicardial dense scar and epicardial electrical heterogeneity. The relative differences in conduction velocity and action potential duration between healthy myocardium and heterogeneous tissue were significantly smaller in the CDC-treated group than in the control group. The lower electrical heterogeneity coincides with heterogeneous tissue with less fibrosis, better cardiomyocyte viability, and a greater quantity and better polarity of connexin 43. At the endocardial level, no differences were detected between groups. Intrapericardial CDCs produce anatomical and functional changes in the epicardial arrhythmic substrate, which could have an anti-arrhythmic effect. Frontiers Media S.A. 2022-11-15 /pmc/articles/PMC9705343/ /pubmed/36457311 http://dx.doi.org/10.3389/fphys.2022.1041348 Text en Copyright © 2022 Carta-Bergaz, Ríos-Muñoz, Crisóstomo, Sánchez-Margallo, Ledesma-Carbayo, Bermejo-Thomas, Fernández-Avilés and Arenal-Maíz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Carta-Bergaz, Alejandro
Ríos-Muñoz, Gonzalo R.
Crisóstomo, Verónica
Sánchez-Margallo, Francisco M.
Ledesma-Carbayo, María J.
Bermejo-Thomas, Javier
Fernández-Avilés, Francisco
Arenal-Maíz, Ángel
Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title_full Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title_fullStr Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title_full_unstemmed Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title_short Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
title_sort intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705343/
https://www.ncbi.nlm.nih.gov/pubmed/36457311
http://dx.doi.org/10.3389/fphys.2022.1041348
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