Cargando…
Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705809/ https://www.ncbi.nlm.nih.gov/pubmed/36123020 http://dx.doi.org/10.1292/jvms.22-0307 |
_version_ | 1784840359829831680 |
---|---|
author | OKAMOTO, Mariko FURUYA, Hirotaka SUGIMOTO, Ikuko TAKAMATSU, Daisuke |
author_facet | OKAMOTO, Mariko FURUYA, Hirotaka SUGIMOTO, Ikuko TAKAMATSU, Daisuke |
author_sort | OKAMOTO, Mariko |
collection | PubMed |
description | American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness. |
format | Online Article Text |
id | pubmed-9705809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-97058092022-12-01 Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays OKAMOTO, Mariko FURUYA, Hirotaka SUGIMOTO, Ikuko TAKAMATSU, Daisuke J Vet Med Sci Bacteriology American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness. The Japanese Society of Veterinary Science 2022-09-19 2022-11 /pmc/articles/PMC9705809/ /pubmed/36123020 http://dx.doi.org/10.1292/jvms.22-0307 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Bacteriology OKAMOTO, Mariko FURUYA, Hirotaka SUGIMOTO, Ikuko TAKAMATSU, Daisuke Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title | Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title_full | Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title_fullStr | Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title_full_unstemmed | Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title_short | Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays |
title_sort | detection of macrolide resistance genes, ermc and ermb, in japanese honey using real-time pcr assays |
topic | Bacteriology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705809/ https://www.ncbi.nlm.nih.gov/pubmed/36123020 http://dx.doi.org/10.1292/jvms.22-0307 |
work_keys_str_mv | AT okamotomariko detectionofmacrolideresistancegenesermcandermbinjapanesehoneyusingrealtimepcrassays AT furuyahirotaka detectionofmacrolideresistancegenesermcandermbinjapanesehoneyusingrealtimepcrassays AT sugimotoikuko detectionofmacrolideresistancegenesermcandermbinjapanesehoneyusingrealtimepcrassays AT takamatsudaisuke detectionofmacrolideresistancegenesermcandermbinjapanesehoneyusingrealtimepcrassays |