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Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays

American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such...

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Autores principales: OKAMOTO, Mariko, FURUYA, Hirotaka, SUGIMOTO, Ikuko, TAKAMATSU, Daisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705809/
https://www.ncbi.nlm.nih.gov/pubmed/36123020
http://dx.doi.org/10.1292/jvms.22-0307
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author OKAMOTO, Mariko
FURUYA, Hirotaka
SUGIMOTO, Ikuko
TAKAMATSU, Daisuke
author_facet OKAMOTO, Mariko
FURUYA, Hirotaka
SUGIMOTO, Ikuko
TAKAMATSU, Daisuke
author_sort OKAMOTO, Mariko
collection PubMed
description American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness.
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spelling pubmed-97058092022-12-01 Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays OKAMOTO, Mariko FURUYA, Hirotaka SUGIMOTO, Ikuko TAKAMATSU, Daisuke J Vet Med Sci Bacteriology American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness. The Japanese Society of Veterinary Science 2022-09-19 2022-11 /pmc/articles/PMC9705809/ /pubmed/36123020 http://dx.doi.org/10.1292/jvms.22-0307 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
OKAMOTO, Mariko
FURUYA, Hirotaka
SUGIMOTO, Ikuko
TAKAMATSU, Daisuke
Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title_full Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title_fullStr Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title_full_unstemmed Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title_short Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays
title_sort detection of macrolide resistance genes, ermc and ermb, in japanese honey using real-time pcr assays
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705809/
https://www.ncbi.nlm.nih.gov/pubmed/36123020
http://dx.doi.org/10.1292/jvms.22-0307
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