Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017

In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV)...

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Autores principales: DONG, Hieu Van, SUZUKI, Maya, TAKEMAE, Hitoshi, JAMSRANSUREN, Dulamjav, MATSUDA, Sachiko, NGUYEN, Hiep Dinh, MIZUTANI, Tetsuya, TAKEDA, Yohei, OGAWA, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705814/
https://www.ncbi.nlm.nih.gov/pubmed/36216530
http://dx.doi.org/10.1292/jvms.22-0096
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author DONG, Hieu Van
SUZUKI, Maya
TAKEMAE, Hitoshi
JAMSRANSUREN, Dulamjav
MATSUDA, Sachiko
NGUYEN, Hiep Dinh
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
author_facet DONG, Hieu Van
SUZUKI, Maya
TAKEMAE, Hitoshi
JAMSRANSUREN, Dulamjav
MATSUDA, Sachiko
NGUYEN, Hiep Dinh
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
author_sort DONG, Hieu Van
collection PubMed
description In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016–2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi.
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spelling pubmed-97058142022-12-01 Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017 DONG, Hieu Van SUZUKI, Maya TAKEMAE, Hitoshi JAMSRANSUREN, Dulamjav MATSUDA, Sachiko NGUYEN, Hiep Dinh MIZUTANI, Tetsuya TAKEDA, Yohei OGAWA, Haruko J Vet Med Sci Virology In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016–2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi. The Japanese Society of Veterinary Science 2022-10-11 2022-11 /pmc/articles/PMC9705814/ /pubmed/36216530 http://dx.doi.org/10.1292/jvms.22-0096 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
DONG, Hieu Van
SUZUKI, Maya
TAKEMAE, Hitoshi
JAMSRANSUREN, Dulamjav
MATSUDA, Sachiko
NGUYEN, Hiep Dinh
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title_full Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title_fullStr Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title_full_unstemmed Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title_short Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017
title_sort viral rna extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in tokachi province, japan, in 2016–2017
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705814/
https://www.ncbi.nlm.nih.gov/pubmed/36216530
http://dx.doi.org/10.1292/jvms.22-0096
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