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Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin
To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe(3)O(4)-NH(2) with tetraethyl silicate (TEOS), mic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Microbiology and Biotechnology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705872/ https://www.ncbi.nlm.nih.gov/pubmed/32627762 http://dx.doi.org/10.4014/jmb.2004.04055 |
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author | Xu, Jin Shi, Xuejia Zhang, Xiaomeng Wang, Zhenzhong Xiao, Wei Zhao, Linguo |
author_facet | Xu, Jin Shi, Xuejia Zhang, Xiaomeng Wang, Zhenzhong Xiao, Wei Zhao, Linguo |
author_sort | Xu, Jin |
collection | PubMed |
description | To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe(3)O(4)-NH(2) with tetraethyl silicate (TEOS), microcrystalline cellulose and zinc nitrate hexahydrate. FSNcZ8 displayed better magnetic stability and higher-temperature stability than unmodified Fe(3)O(4)-NH(2) (FN), and it was used to adsorb and immobilize TpeRha from Thermotoga petrophilea 13995. As for properties, FSNcZ8-TpeRha showed optimal reaction temperature and pH of 90°C and 5.0, while its highest activity approached 714 U/g. In addition, FSNcZ8-TpeRha had better higher-temperature stability than FN. After incubation at 80°C for 3 h, the residual enzyme activities of FSNcZ8-TpeRha, FN-TpeRha and free enzyme were 93.5%, 63.32%, and 62.77%, respectively. The organic solvent tolerance and the monosaccharides tolerance of FSNcZ8-TpeRha, compared with free TpeRha, were greatly improved. Using naringin (1 mmol/l) as the substrate, the optimal conversion conditions were as follows: FSNcZ8-TpeRha concentration was 6 U/ml; induction temperature was 80°C; the pH was 5.5; induction time was 30 min, and the yield of products was the same as free enzyme. After repeating the reaction 10 times, the conversion of naringin remained above 80%, showing great improvement of the catalytic efficiency and repeated utilization of the immobilized α-L-rhamnosidase. |
format | Online Article Text |
id | pubmed-9705872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97058722022-12-13 Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin Xu, Jin Shi, Xuejia Zhang, Xiaomeng Wang, Zhenzhong Xiao, Wei Zhao, Linguo J Microbiol Biotechnol Research article To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe(3)O(4)-NH(2) with tetraethyl silicate (TEOS), microcrystalline cellulose and zinc nitrate hexahydrate. FSNcZ8 displayed better magnetic stability and higher-temperature stability than unmodified Fe(3)O(4)-NH(2) (FN), and it was used to adsorb and immobilize TpeRha from Thermotoga petrophilea 13995. As for properties, FSNcZ8-TpeRha showed optimal reaction temperature and pH of 90°C and 5.0, while its highest activity approached 714 U/g. In addition, FSNcZ8-TpeRha had better higher-temperature stability than FN. After incubation at 80°C for 3 h, the residual enzyme activities of FSNcZ8-TpeRha, FN-TpeRha and free enzyme were 93.5%, 63.32%, and 62.77%, respectively. The organic solvent tolerance and the monosaccharides tolerance of FSNcZ8-TpeRha, compared with free TpeRha, were greatly improved. Using naringin (1 mmol/l) as the substrate, the optimal conversion conditions were as follows: FSNcZ8-TpeRha concentration was 6 U/ml; induction temperature was 80°C; the pH was 5.5; induction time was 30 min, and the yield of products was the same as free enzyme. After repeating the reaction 10 times, the conversion of naringin remained above 80%, showing great improvement of the catalytic efficiency and repeated utilization of the immobilized α-L-rhamnosidase. Korean Society for Microbiology and Biotechnology 2021-03-28 2020-06-24 /pmc/articles/PMC9705872/ /pubmed/32627762 http://dx.doi.org/10.4014/jmb.2004.04055 Text en Copyright © 2021 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research article Xu, Jin Shi, Xuejia Zhang, Xiaomeng Wang, Zhenzhong Xiao, Wei Zhao, Linguo Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title | Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title_full | Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title_fullStr | Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title_full_unstemmed | Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title_short | Immobilization of GH78 ⓹-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe(3)O(4)-SiO(2)-NH(2)-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin |
title_sort | immobilization of gh78 ⓹-l-rhamnosidase from thermotoga petrophilea with high-temperature-resistant magnetic particles fe(3)o(4)-sio(2)-nh(2)-cellu-zif8 and its application in the production of prunin form naringin |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705872/ https://www.ncbi.nlm.nih.gov/pubmed/32627762 http://dx.doi.org/10.4014/jmb.2004.04055 |
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