Cargando…
Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation
Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Microbiology and Biotechnology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705882/ https://www.ncbi.nlm.nih.gov/pubmed/34489373 http://dx.doi.org/10.4014/jmb.2107.07023 |
_version_ | 1784840379631140864 |
---|---|
author | Min, Jae-Hong Kim, Seong-Man Park, JI-Won Kwon, Nam Hoon Goo, Soo Hyeon Ngatinem, Ningsih, Sri Paik, Jin-Hyub Choi, Sangho Oh, Sei-Ryang Han, Sang-Bae Ahn, Kyung-Seop Lee, Jae-Won |
author_facet | Min, Jae-Hong Kim, Seong-Man Park, JI-Won Kwon, Nam Hoon Goo, Soo Hyeon Ngatinem, Ningsih, Sri Paik, Jin-Hyub Choi, Sangho Oh, Sei-Ryang Han, Sang-Bae Ahn, Kyung-Seop Lee, Jae-Won |
author_sort | Min, Jae-Hong |
collection | PubMed |
description | Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. The aforementioned findings collectively indicate that LOLE exerts an ameliorative effect on inflammatory response in the airway of ALI mice. |
format | Online Article Text |
id | pubmed-9705882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97058822022-12-13 Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation Min, Jae-Hong Kim, Seong-Man Park, JI-Won Kwon, Nam Hoon Goo, Soo Hyeon Ngatinem, Ningsih, Sri Paik, Jin-Hyub Choi, Sangho Oh, Sei-Ryang Han, Sang-Bae Ahn, Kyung-Seop Lee, Jae-Won J Microbiol Biotechnol Research article Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. The aforementioned findings collectively indicate that LOLE exerts an ameliorative effect on inflammatory response in the airway of ALI mice. The Korean Society for Microbiology and Biotechnology 2021-11-28 2021-09-03 /pmc/articles/PMC9705882/ /pubmed/34489373 http://dx.doi.org/10.4014/jmb.2107.07023 Text en Copyright © 2021 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research article Min, Jae-Hong Kim, Seong-Man Park, JI-Won Kwon, Nam Hoon Goo, Soo Hyeon Ngatinem, Ningsih, Sri Paik, Jin-Hyub Choi, Sangho Oh, Sei-Ryang Han, Sang-Bae Ahn, Kyung-Seop Lee, Jae-Won Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title | Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title_full | Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title_fullStr | Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title_full_unstemmed | Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title_short | Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation |
title_sort | lagerstroemia ovalifolia exerts anti-inflammatory effects in mice of lpsinduced ali via downregulating of mapk and nf-κb activation |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705882/ https://www.ncbi.nlm.nih.gov/pubmed/34489373 http://dx.doi.org/10.4014/jmb.2107.07023 |
work_keys_str_mv | AT minjaehong lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT kimseongman lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT parkjiwon lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT kwonnamhoon lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT goosoohyeon lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT ngatinem lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT ningsihsri lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT paikjinhyub lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT choisangho lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT ohseiryang lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT hansangbae lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT ahnkyungseop lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation AT leejaewon lagerstroemiaovalifoliaexertsantiinflammatoryeffectsinmiceoflpsinducedaliviadownregulatingofmapkandnfkbactivation |