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An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyc...

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Detalles Bibliográficos
Autores principales: Dong, Jianxin, Wei, Jiaxiu, Li, Han, Zhao, Shiyao, Guan, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705919/
https://www.ncbi.nlm.nih.gov/pubmed/34489377
http://dx.doi.org/10.4014/jmb.2106.06083
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author Dong, Jianxin
Wei, Jiaxiu
Li, Han
Zhao, Shiyao
Guan, Wenjun
author_facet Dong, Jianxin
Wei, Jiaxiu
Li, Han
Zhao, Shiyao
Guan, Wenjun
author_sort Dong, Jianxin
collection PubMed
description The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.
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spelling pubmed-97059192022-12-13 An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces Dong, Jianxin Wei, Jiaxiu Li, Han Zhao, Shiyao Guan, Wenjun J Microbiol Biotechnol Research article The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces. The Korean Society for Microbiology and Biotechnology 2021-12-28 2021-09-04 /pmc/articles/PMC9705919/ /pubmed/34489377 http://dx.doi.org/10.4014/jmb.2106.06083 Text en Copyright © 2021 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Dong, Jianxin
Wei, Jiaxiu
Li, Han
Zhao, Shiyao
Guan, Wenjun
An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title_full An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title_fullStr An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title_full_unstemmed An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title_short An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
title_sort efficient markerless deletion system suitable for the industrial strains of streptomyces
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705919/
https://www.ncbi.nlm.nih.gov/pubmed/34489377
http://dx.doi.org/10.4014/jmb.2106.06083
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