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Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates

A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified...

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Autores principales: Liers, Christiane, Ullrich, René, Kellner, Harald, Chi, Do Huu, Quynh, Dang Thu, Luyen, Nguyen Dinh, Huong, Le Mai, Hofrichter, Martin, Nghi, Do Huu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705965/
https://www.ncbi.nlm.nih.gov/pubmed/34409952
http://dx.doi.org/10.4014/jmb.2106.06053
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author Liers, Christiane
Ullrich, René
Kellner, Harald
Chi, Do Huu
Quynh, Dang Thu
Luyen, Nguyen Dinh
Huong, Le Mai
Hofrichter, Martin
Nghi, Do Huu
author_facet Liers, Christiane
Ullrich, René
Kellner, Harald
Chi, Do Huu
Quynh, Dang Thu
Luyen, Nguyen Dinh
Huong, Le Mai
Hofrichter, Martin
Nghi, Do Huu
author_sort Liers, Christiane
collection PubMed
description A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified structures. The contribution of XpoGH78 to the conversion of lignocellulosic materials without any chemical pretreatment to release the water-soluble aromatic fragments, carbohydrates, and methanol was studied. The disintegrating effect of enzymatic lignocellulose treatment can be significantly improved by using different kinds of hydrolases and phenoloxidases. The considerable changes in low (3 kDa), medium (30 kDa), and high (> 200 kDa) aromatic fragments were observed after the treatment with XpoGH78 alone or with this potent cocktail. Synergistic conversion of rape straw also resulted in a release of 17.3 mg of total carbohydrates (e.g., arabinose, galactose, glucose, mannose, xylose) per gram of substrate after incubating for 72 h. Moreover, the treatment of rape straw with XpoGH78 led to a marginal methanol release of approximately 17 μg/g and improved to 270 μg/g by cooperation with the above accessory enzymes. In the case of beech wood conversion, the combined catalysis by XpoGH78 and laccase caused an effect comparable with that of fungal strain X. polymorpha in woody cultures concerning the liberation of aromatic lignocellulose fragments.
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spelling pubmed-97059652022-12-13 Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates Liers, Christiane Ullrich, René Kellner, Harald Chi, Do Huu Quynh, Dang Thu Luyen, Nguyen Dinh Huong, Le Mai Hofrichter, Martin Nghi, Do Huu J Microbiol Biotechnol Research article A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified structures. The contribution of XpoGH78 to the conversion of lignocellulosic materials without any chemical pretreatment to release the water-soluble aromatic fragments, carbohydrates, and methanol was studied. The disintegrating effect of enzymatic lignocellulose treatment can be significantly improved by using different kinds of hydrolases and phenoloxidases. The considerable changes in low (3 kDa), medium (30 kDa), and high (> 200 kDa) aromatic fragments were observed after the treatment with XpoGH78 alone or with this potent cocktail. Synergistic conversion of rape straw also resulted in a release of 17.3 mg of total carbohydrates (e.g., arabinose, galactose, glucose, mannose, xylose) per gram of substrate after incubating for 72 h. Moreover, the treatment of rape straw with XpoGH78 led to a marginal methanol release of approximately 17 μg/g and improved to 270 μg/g by cooperation with the above accessory enzymes. In the case of beech wood conversion, the combined catalysis by XpoGH78 and laccase caused an effect comparable with that of fungal strain X. polymorpha in woody cultures concerning the liberation of aromatic lignocellulose fragments. The Korean Society for Microbiology and Biotechnology 2021-10-28 2021-08-20 /pmc/articles/PMC9705965/ /pubmed/34409952 http://dx.doi.org/10.4014/jmb.2106.06053 Text en Copyright © 2021 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Liers, Christiane
Ullrich, René
Kellner, Harald
Chi, Do Huu
Quynh, Dang Thu
Luyen, Nguyen Dinh
Huong, Le Mai
Hofrichter, Martin
Nghi, Do Huu
Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title_full Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title_fullStr Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title_full_unstemmed Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title_short Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates
title_sort bioconversion of lignocellulosic materials with the contribution of a multifunctional gh78 glycoside hydrolase from xylaria polymorpha to release aromatic fragments and carbohydrates
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705965/
https://www.ncbi.nlm.nih.gov/pubmed/34409952
http://dx.doi.org/10.4014/jmb.2106.06053
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