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In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows

Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO)...

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Autores principales: Buldain, Daniel, Gortari Castillo, Lihuel, Buchamer, Andrea Verónica, Bandoni, Arnaldo, Marchetti, Laura, Mestorino, Nora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705979/
https://www.ncbi.nlm.nih.gov/pubmed/36458057
http://dx.doi.org/10.3389/fvets.2022.1005616
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author Buldain, Daniel
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo
Marchetti, Laura
Mestorino, Nora
author_facet Buldain, Daniel
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo
Marchetti, Laura
Mestorino, Nora
author_sort Buldain, Daniel
collection PubMed
description Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
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spelling pubmed-97059792022-11-30 In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows Buldain, Daniel Gortari Castillo, Lihuel Buchamer, Andrea Verónica Bandoni, Arnaldo Marchetti, Laura Mestorino, Nora Front Vet Sci Veterinary Science Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus. Frontiers Media S.A. 2022-11-15 /pmc/articles/PMC9705979/ /pubmed/36458057 http://dx.doi.org/10.3389/fvets.2022.1005616 Text en Copyright © 2022 Buldain, Gortari Castillo, Buchamer, Bandoni, Marchetti and Mestorino. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Buldain, Daniel
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo
Marchetti, Laura
Mestorino, Nora
In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_full In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_fullStr In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_full_unstemmed In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_short In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_sort in vitro synergistic interaction between melaleuca armillaris essential oil and erythromycin against staphylococcus aureus isolated from dairy cows
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705979/
https://www.ncbi.nlm.nih.gov/pubmed/36458057
http://dx.doi.org/10.3389/fvets.2022.1005616
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